CAJ | 학술논문

利用高效液相色谱-串联质谱（HPLC-MS/MS）同时测定食用油中16种抗氧化剂。食用油中的抗氧化剂经正己烷溶解、含L-抗坏血酸棕榈酸盐的乙腈萃取后，经C18柱分离，乙腈-0.1%甲酸溶液体系为流动相进行梯度洗脱，多反应监测模式（MRM）检测，外标法定量。16种抗氧化剂呈良好的线性关系（r2=0.9912～0.9988），方法的测定低限为0.1～0.5 mg/kg，在测定低限浓度水平下的平均回收率为78.6%～101.2%，相对标准偏差为3.50%～13.19%（n=10）。该方法准确、灵敏、重现性好，可用于食用油中16种抗氧化剂的检测。
이용고효액상색보-천련질보（HPLC-MS/MS）동시측정식용유중16충항양화제。식용유중적항양화제경정기완용해、함L-항배혈산종려산염적을정췌취후，경C18주분리，을정-0.1%갑산용액체계위류동상진행제도세탈，다반응감측모식（MRM）검측，외표법정량。16충항양화제정량호적선성관계（r2=0.9912～0.9988），방법적측정저한위0.1～0.5 mg/kg，재측정저한농도수평하적평균회수솔위78.6%～101.2%，상대표준편차위3.50%～13.19%（n=10）。해방법준학、령민、중현성호，가용우식용유중16충항양화제적검측。
A method was developed for the simultaneous determination of 16 antioxidants in edible oil using high performance liquid chromatography-tandem mass spectrometry. The antioxidants in the sample were dissolved with hexane, extracted with acetonitrile containing ascorbyl palpitate, separated on a reversed-phase C18 column with a mobile phase of acetonitrile-water containing 0.1%formic acid by gradient elution. The detection was performed by MS/MS under multiple reaction monitoring(MRM)mode, and the quantitation was carried out by the external standard method. The calibration curves showed good linearities for 16 antioxidants with correlation coefficients of 0.991 2-0.998 8. The determination limits of 16 antioxidants were between 0.1 mg/kg and 0.5 mg/kg. Sample were fortifid at the level of the limit of determination with the antioxidants mixed standard solution. The recoveries were between 78.6%and 101.2%, and the relative standard deviations of repeatability were between 3.50%and 13.19%(n=10). The method is reliable, sensitive and reproducible, and can be applied in the determination of 16 antioxidants in edible oil.