CAJ | 학술논문

微生物发酵饲料作为一种新型蛋白质饲料成为当前研究热点。试验以核桃压榨后的核桃饼粕为主要原料，混合玉米粉接种酵母菌进行固态发酵，研究发酵产物粗蛋白含量的变化情况，以期获得新型高蛋白含量的微生物发酵饲料。以粗蛋白含量为指标，通过单因素试验和正交试验确定发酵的最佳条件。结果表明，发酵最佳条件为：玉米粉和核桃饼粕配比2：1、接种量10％、发酵时间4天、发酵温度30℃，在此条件下发酵得到的饲料粗蛋白含量达到34.26％，与未发酵的玉米粉粗蛋白含量10.78％相比，蛋白含量提高了23.48％。得出的结论有利于人们对核桃饼粕的开发利用，又为发酵植物蛋白生产高蛋白饲料研究提供基础和依据。
미생물발효사료작위일충신형단백질사료성위당전연구열점。시험이핵도압자후적핵도병박위주요원료，혼합옥미분접충효모균진행고태발효，연구발효산물조단백함량적변화정황，이기획득신형고단백함량적미생물발효사료。이조단백함량위지표，통과단인소시험화정교시험학정발효적최가조건。결과표명，발효최가조건위：옥미분화핵도병박배비2：1、접충량10％、발효시간4천、발효온도30℃，재차조건하발효득도적사료조단백함량체도34.26％，여미발효적옥미분조단백함량10.78％상비，단백함량제고료23.48％。득출적결론유리우인문대핵도병박적개발이용，우위발효식물단백생산고단백사료연구제공기출화의거。
As a new type of protein feed , microbial fermented feed became a research hot spot.The pressed walnut cake was used as the main raw material and mixed with corn flour to be inoculated with yeast for solid state fermentation , aiming at studying the change of crude protein content in the fermentation product in order to obtain new microbial fermented feed with high protein content.Considering crude protein content as the indicator , the optimal fermentation conditions were determined by single factor experiment and orthogonal experiment.The results showed that the optimal fermentation conditions were as follows: corn flour and walnut cake ratio of 2:1, inoculum concentration of 10%, fermentation time of four days and fermentation temperature of 30℃.Under such conditions , the crude protein content in the feed by fermentation was 34.26%.Compared with unfermented corn flour with crude protein content of 10.78%, it was improved by 23.48%.The conclusion not only contributed to the development and utilization of walnut cake but also provided the foundation and basis for the study of production of high -protein feed by fermentation plant protein.