CAJ | 학술논문

目的：使用蛋白质做稳定剂来合成银纳米粒子，并探讨其稳定性，为其在生物医学领域及日常生活中的应用提供理论指导。方法采用化学还原法一步合成溶菌酶稳定的银纳米粒子，改变稳定性影响因素并观察银纳米溶胶的变化。即加入不同浓度的氯化钠来改变银纳米溶胶的离子强度，用酸或碱来调节溶胶pH，对溶胶进行多次冷冻-解冻循环。并且用柠檬酸稳定的银纳米粒子做对照试验。结果溶菌酶稳定的银纳米粒子对离子强度的变化比较敏感，在10 mmol/L NaCl条件下完全沉淀；在酸性条件下能够稳定存在（pH≤6）；冷冻-解冻循环对其稳定性影响比较小。结论溶菌酶稳定的银纳米粒子的稳定性受周围环境影响较大，在使用及储存时需要加以注意。
목적：사용단백질주은정제래합성은납미입자，병탐토기은정성，위기재생물의학영역급일상생활중적응용제공이론지도。방법채용화학환원법일보합성용균매은정적은납미입자，개변은정성영향인소병관찰은납미용효적변화。즉가입불동농도적록화납래개변은납미용효적리자강도，용산혹감래조절용효pH，대용효진행다차냉동-해동순배。병차용저몽산은정적은납미입자주대조시험。결과용균매은정적은납미입자대리자강도적변화비교민감，재10 mmol/L NaCl조건하완전침정；재산성조건하능구은정존재（pH≤6）；냉동-해동순배대기은정성영향비교소。결론용균매은정적은납미입자적은정성수주위배경영향교대，재사용급저존시수요가이주의。
Objective We have synthesized silver nanoparticles using protein as the stabilizer and discussed their stability to provide theoretical guidance for their application in biomedical ifeld and daily life. Methods We have presented a one-step approach of chemical reduction to prepare the lysozyme-stabilized silver nanoparticles. Results The stability of lysozyme-stabilized silver nanoparticles was sensitive to the change of ionic strength and the nanoparticles precipitated completely in 10 mmol/L NaCl. The lysozyme-stabilized silver nanoparticles were stable under acidic condition (pH≤6). And the freeze-thawing cycle had a little inlfuence on their stability. Conclusion The stability of lysozyme-stabilized silver nanoparticles is greatly inlfuenced by the surrounding conditions. The variation caused by the stability factors should be considered for use and storage of the lysozyme-stabilized silver nanoparticles.