CAJ | 학술논문

[目的]分析白桦金属硫蛋白的生物信息学信息，为后续基因重要功能研究奠定基础。[方法]利用生物信息学方法和工具对白桦（Betula platyphylla）金属硫蛋白（metallothion-ein，MT）的理化性质、跨膜结构、疏水性/亲水性、二级结构、结构功能域、功能分类进行了分析和预测。[结果]金属硫蛋白的分子量为7.75 kD，等电点为5.66，中部有较强疏水区，而其两端为明显亲水区，其可能为非跨膜蛋白，主要构件为卷曲，属于 Metallothio_2蛋白家族。[结论]该研究可为白桦金属硫蛋白基因功能研究提供参考。
[목적]분석백화금속류단백적생물신식학신식，위후속기인중요공능연구전정기출。[방법]이용생물신식학방법화공구대백화（Betula platyphylla）금속류단백（metallothion-ein，MT）적이화성질、과막결구、소수성/친수성、이급결구、결구공능역、공능분류진행료분석화예측。[결과]금속류단백적분자량위7.75 kD，등전점위5.66，중부유교강소수구，이기량단위명현친수구，기가능위비과막단백，주요구건위권곡，속우 Metallothio_2단백가족。[결론]해연구가위백화금속류단백기인공능연구제공삼고。
[Objective] This study aimed to analyze the bioinformatics information of Betula phatyphyl a metal othionein for subsequent investigation of the important func-tions of metal othionein gene. [Method] The physical and chemical properties, trans-membrane domains, hydrophobicity / hydrophilicity, secondary structure, functional domains and functional classification of B. phatyphyl a metal othionein were analyzed and predicted using bioinformatics methods and tools. [Result] The relative molecular mass of B. phatyphyl a metal othionein is 7.75 kD and isoelectric point is 5.66; there is a strong hydrophobic region in the center of B. phatyphyl a metal othionein and hydrophilic regions in both ends; B. phatyphyl a metal othionein may be a non-trans-membrane protein and is mainly constituted by random coil without enzymatic activi-ty, suggesting that it belongs to Metal othio_2 protein family. [Conclusion] This study provided reference for further functional research of B. phatyphyl a metal othionein gene.