CAJ | 학술논문

[目的]探讨 IGFs系统对鹅骨骼肌生长的影响。[方法]以太湖鹅和皖西白鹅为研究材料，采用荧光定量 PCR方法研究鹅70日龄腿肌中 GHR、IGF-I、IGF-IR和 IGFBP-3 mRNA表达的品种、性别特异性，并与屠宰性能做相关性分析。[结果]结果表明，鹅腿肌 GHR、IGF-I、IGF-IR和 IGFBP-3 mRNA表达没有品种差异性，而体重和腿肌重品种差异显著。体重、腿肌重和腿肌率均无性别差异，除了太湖鹅腿肌 IGF-I mRNA 表达公鹅显著大于母鹅（P=0.032），其他3个基因均无性别差异。检测的4个基因中仅IGFBP-3 mRNA表达和腿肌率呈极显著正相关，提示70日龄时 IGFs系统可能通过 IGFBP-3发挥对鹅腿肌生长的调节作用。[结论]为鹅骨骼肌生长发育研究工作提供理论依据。
[목적]탐토 IGFs계통대아골격기생장적영향。[방법]이태호아화환서백아위연구재료，채용형광정량 PCR방법연구아70일령퇴기중 GHR、IGF-I、IGF-IR화 IGFBP-3 mRNA표체적품충、성별특이성，병여도재성능주상관성분석。[결과]결과표명，아퇴기 GHR、IGF-I、IGF-IR화 IGFBP-3 mRNA표체몰유품충차이성，이체중화퇴기중품충차이현저。체중、퇴기중화퇴기솔균무성별차이，제료태호아퇴기 IGF-I mRNA 표체공아현저대우모아（P=0.032），기타3개기인균무성별차이。검측적4개기인중부IGFBP-3 mRNA표체화퇴기솔정겁현저정상관，제시70일령시 IGFs계통가능통과 IGFBP-3발휘대아퇴기생장적조절작용。[결론]위아골격기생장발육연구공작제공이론의거。
[Objective] This study aimed to explore the effects of IGFs system on the growth of goose skeletal muscles. [Method] Quantitative fluorescence PCR technique was adopted to study the variety- and gender-specificity in the expression of GHR, IGF-I, IGF-IR and IGFBP-3 genes in leg muscles of 70-day-old Taihu goose and Wanxi white goose, and the correlation between their expression and carcass traits was also investigated. [Result] There was no variety difference in the expression of GHR, IGF-I, IGF-IR and IGFBP-3 genes in leg muscles of Taihu goose and Wanxi white goose, but there were significant variety differences in the body weight and leg muscle weight. There were no gender difference in the body weight, leg muscle weight and the rate of leg muscles; except IGF-I mRNA level that was significantly higher in male Taihu goose than in female ones(P=0.032), there was no gender dif-ference in the expression of other three genes. Among the four tested genes, only IGFBP-3 mRNA exhibited an extremely significantly positively correlation with the rate of leg muscles, suggesting that IGFs may play a role in regulating the growth of leg muscles via IGFBP-3 system in 70-day-old goose. [Conclusion] This study provides theoretical basis for research in the skeletal growth and development.