CAJ | 학술논문

[目的]研究不同生长素对绣球丁香扦插生根的影响及生根过程中相关酶活性的变化。[方法]以绣球丁香为材料，选用 IBA、ABT、NAA 3种生长素分别于500、1000、1500、2000 mg/L浓度下进行嫩枝扦插对比试验，研究生根过程中过氧化物酶（POD）、多酚氧化酶（PPO）和吲哚乙酸氧化酶（IAAO）的活性变化。[结果]IBA、ABT、NAA 3种生长素的最适浓度分别为1500、1000、1000 mg/L，其中以IBA 1500 mg/L处理效果最佳。在整个生根过程中，处理组与对照组 POD与 PPO活性动态变化趋势基本一致，但处理组的 POD与PPO活性显著提高且变化幅度更大；扦插初期对照组与处理组的 IAAO活性呈相反的变化趋势，前者缓慢升高而后者迅速下降，且处理组显著低于对照组；此外，较高活性的 POD与PPO利于不定根的诱导，较低活性的 IAAO则利于不定根的发育与伸长。[结论]该研究初步揭示了绣球丁香嫩枝扦插的生根机理。
[목적]연구불동생장소대수구정향천삽생근적영향급생근과정중상관매활성적변화。[방법]이수구정향위재료，선용 IBA、ABT、NAA 3충생장소분별우500、1000、1500、2000 mg/L농도하진행눈지천삽대비시험，연구생근과정중과양화물매（POD）、다분양화매（PPO）화신타을산양화매（IAAO）적활성변화。[결과]IBA、ABT、NAA 3충생장소적최괄농도분별위1500、1000、1000 mg/L，기중이IBA 1500 mg/L처리효과최가。재정개생근과정중，처리조여대조조 POD여 PPO활성동태변화추세기본일치，단처리조적 POD여PPO활성현저제고차변화폭도경대；천삽초기대조조여처리조적 IAAO활성정상반적변화추세，전자완만승고이후자신속하강，차처리조현저저우대조조；차외，교고활성적 POD여PPO리우불정근적유도，교저활성적 IAAO칙리우불정근적발육여신장。[결론]해연구초보게시료수구정향눈지천삽적생근궤리。
[Objective] This study aimed to investigate the effects of different exoge-nous hormones on the rooting of Syringa microphyl a cuttings and the change in related enzymes activity during the rooting process. [Method] Three different exoge-nous hormones IBA, NAA and ABT, each with concentrations of 500, 1 000, 1 500 and 2 000 mg/L were used to treat S. microphyl a cuttings, and changes in the ac-tivities of peroxidase (POD), polyphenol oxidase (PPO) and indoleacetic acid oxidase (IAAO) during the rooting process were also investigated. [Result] The most appro-priate concentrations of IBA, ABT and NAA were 1 500, 1 000 and 1 000 mg/L, respectively, and the 1 500 mg/L IBA treatment exhibited the best effect on rooting. Throughout the rooting process, POD and PPO activities showed the same trends in the treatment groups as those in the control group, but the POD and PPO activi-ties in the treatment groups were increased significantly, with greater amplitude of variation; at the early stage, IAAO activity exhibited an opposite trend between the control group and the treatment groups, which increased slowly in the former, but decreased rapidly in the latter, and it was significantly lower in the treatment groups compared to the control; additional y, higher POD and IAAO activities were con-ducive to the induction of adventitious roots, and lower POD and IAAO activities fa-vored their formation and elongation. [Conclusion] This study has preliminarily clari-fied the rooting mechanism of S. microphyl a cuttings.