CAJ | 학술논문

[目的]用 RSAP标记技术分析中国境内分布的33种野生石斛的遗传多样性及亲缘关系。[方法]建立石斛 RSAP-PCR反应体系，从45对 RSAP引物中筛选出33对多态性引物，对33种野生石斛的基因组 DNA进行 PCR扩增。[结果]这33对引物共扩增出2047条带，其中多态性条带为2044条，多态性比例99.8%，平均每对引物产生61.9条多态性条带。材料间遗传相似系数变化范围为0.081-0.442，说明本实验所测试的材料具有较广的遗传基础。根据 RSAP 标记的结果，采用UPGMA法进行聚类分析，将33种野生石斛分为6类。结果表明剑叶石斛和滇桂石斛之间亲缘关系最近，而华石斛与喇叭唇石斛、细叶石斛之间亲缘关系最远。[结论]石斛具有丰富的遗传多样性，该研究可为石斛属的分类、育种及杂交亲本的选择提供分子水平依据。
[목적]용 RSAP표기기술분석중국경내분포적33충야생석곡적유전다양성급친연관계。[방법]건립석곡 RSAP-PCR반응체계，종45대 RSAP인물중사선출33대다태성인물，대33충야생석곡적기인조 DNA진행 PCR확증。[결과]저33대인물공확증출2047조대，기중다태성조대위2044조，다태성비례99.8%，평균매대인물산생61.9조다태성조대。재료간유전상사계수변화범위위0.081-0.442，설명본실험소측시적재료구유교엄적유전기출。근거 RSAP 표기적결과，채용UPGMA법진행취류분석，장33충야생석곡분위6류。결과표명검협석곡화전계석곡지간친연관계최근，이화석곡여나팔진석곡、세협석곡지간친연관계최원。[결론]석곡구유봉부적유전다양성，해연구가위석곡속적분류、육충급잡교친본적선택제공분자수평의거。
[Objective] This study aimed to analyse the genetic diversity of and genet-ic relationship between 33 Dendrobium species by using RSAP (Restriction Site Amplification Polymorphism) marker technique. [Method] A PCR-reaction system was established, by which 33 pairs of primers screened from 45 pairs were used to con-duct the PCR amplification. [Results] The 33 pairs of primers produced 2 047 ampli-fied fragments, of which 2 044 were polymorphic, with a polymorphism rate of 99.8%, and each pair of primers produced 61.9 polymorphic fragments on average. The genetic similarity coefficients between species ranged from 0.081 to 0.442, sug-gesting that the experimental materials used in this study possess a wide genetic background. According to the result of RSAP, the 33 Dendrobium species were di-vided into 6 groups by the cluster analysis (UPGMA). The result showed that the genetic relationship between D. acinaciforme and D. guangxiense was the closest, and that between D. sinense and either D. lituiflorum or D. hancocki was the fur-thest. [Conclusion] The result suggested that Dendrobium germplasms have a great genetic diversity. This study provides a molecular basis for the classification and breeding of Dendrobium plants, as wel as the selection of hybridization parents in the future.