农业科学与技术（英文版）
農業科學與技術（英文版）
농업과학여기술（영문판）
AGRICULTURAL SCIENCE & TECHNOLOGY
2013年
12期
1702-1706
,共5页
铁皮石斛%原球茎%铬
鐵皮石斛%原毬莖%鉻
철피석곡%원구경%락
Dendrobium candidum%Protocorm%Chromium
[目的]为优化铁皮石斛原球茎富铬培养工艺。[方法]通过Plackett-Burman 试验对时间、温度、培养基铬浓度、NAA浓度、KT浓度和光照强度等因素进行考察，结果显示时间、培养基铬浓度和光照强度3个因素具有显著效应；接着，通过显著因素的最陡爬坡试验，逼近各因素最佳值区域；最后，进行响应面优化分析。[结果]最佳富铬条件为铬浓度0.37 mg/L、培养时间59 d、光照强度1822.22 lx。原球茎铬浓度的预测值为5.08 mg/kg。[结论]响应面法能对铁皮石斛原球茎富铬培养条件进行优化分析，获得最佳工艺参数，为进一步的研究奠定基础。
[目的]為優化鐵皮石斛原毬莖富鉻培養工藝。[方法]通過Plackett-Burman 試驗對時間、溫度、培養基鉻濃度、NAA濃度、KT濃度和光照彊度等因素進行攷察，結果顯示時間、培養基鉻濃度和光照彊度3箇因素具有顯著效應；接著，通過顯著因素的最陡爬坡試驗，逼近各因素最佳值區域；最後，進行響應麵優化分析。[結果]最佳富鉻條件為鉻濃度0.37 mg/L、培養時間59 d、光照彊度1822.22 lx。原毬莖鉻濃度的預測值為5.08 mg/kg。[結論]響應麵法能對鐵皮石斛原毬莖富鉻培養條件進行優化分析，穫得最佳工藝參數，為進一步的研究奠定基礎。
[목적]위우화철피석곡원구경부락배양공예。[방법]통과Plackett-Burman 시험대시간、온도、배양기락농도、NAA농도、KT농도화광조강도등인소진행고찰，결과현시시간、배양기락농도화광조강도3개인소구유현저효응；접착，통과현저인소적최두파파시험，핍근각인소최가치구역；최후，진행향응면우화분석。[결과]최가부락조건위락농도0.37 mg/L、배양시간59 d、광조강도1822.22 lx。원구경락농도적예측치위5.08 mg/kg。[결론]향응면법능대철피석곡원구경부락배양조건진행우화분석，획득최가공예삼수，위진일보적연구전정기출。
[Objective] To optimize the techniques for culturing Cr-enriched Dendrobi-um candidum protocorm. [Method] By adopting Plackett-Burman test, the effects of time, temperature, Cr-content in medium, NAA content, KT content and light intensi-ty on Cr-enriched protocorm were researched. Three factors of time, Cr-content in medium and light intensity had statistical y significant effects; then steepest ascent procedures were applied to define optimal response region of these three factors; fi-nal y the optimal factors were determined by RSM analysis. [Result] The results showed that the optimal conditions for Cr-enrichment were chromium concentration at 0.37 mg/L, culture time of 59 d and il umination intensity of 1 822.22 lx. The predicted value of Cr-content in protocorm was 5.08 mg/kg. [Conclusion] RSM can optimize the techniques for culturing Cr-enriched protocorm of Dendrobium can-didum, provide the optimum process parameters and lay the foundation for further research.
