CAJ | 학술논문

目的：观察抗氧化剂N-乙酰半胱氨酸（NAC）对辐射致小鼠骨髓单个核细胞（BMMNCs）损伤的保护作用，进一步探讨其可能的作用机制。方法实验分为对照组、辐射组（辐射剂量1、4 Gy）、辐射加NAC组（辐射1、4 Gy前30 min各组加入NAC）。按分组不同将2×106/mL浓度细胞悬液与RPMI-1640培养基和2×10-5 mol/L NAC溶液加入2 mL无菌EP管内，37℃CO2孵箱内孵育30 min后γ射线分别辐射1、4 Gy。生物发光法检测小鼠BMMNCs细胞活力、活性氧检测探针（DCFH-DA）检测细胞内活性氧（ROS）水平、集落形成数目检测克隆形成能力。结果辐射暴露4 Gy后，辐射组小鼠BMMNCs细胞活力（发光值：284296.7±16541.2），明显低于对照组的（848586.7±61404.4）；辐射暴露1 Gy后，辐射组细胞内ROS水平（荧光值：6750.0±103.5）高于对照组的（5710.7±56.2），小鼠BMMNCs克隆形成能力辐射组为（626.7±51.3）克隆数/105细胞，低于对照组的（986.7±100.7）克隆数/105细胞；而辐射加NAC组，与辐射组比较小鼠BMMNCs细胞活力增加至发光值352770.0±23466.1、细胞内ROS水平下降至荧光值5430.0±61.0、BMMNCs克隆形成能力增加至（773.3±49.3）克隆数/105细胞。结论 NAC对辐射暴露小鼠BMMNCs损伤可能是通过降低细胞内ROS水平达到保护作用。NAC可以作为后续实验研究的阳性对照药。
목적：관찰항양화제N-을선반광안산（NAC）대복사치소서골수단개핵세포（BMMNCs）손상적보호작용，진일보탐토기가능적작용궤제。방법실험분위대조조、복사조（복사제량1、4 Gy）、복사가NAC조（복사1、4 Gy전30 min각조가입NAC）。안분조불동장2×106/mL농도세포현액여RPMI-1640배양기화2×10-5 mol/L NAC용액가입2 mL무균EP관내，37℃CO2부상내부육30 min후γ사선분별복사1、4 Gy。생물발광법검측소서BMMNCs세포활력、활성양검측탐침（DCFH-DA）검측세포내활성양（ROS）수평、집락형성수목검측극륭형성능력。결과복사폭로4 Gy후，복사조소서BMMNCs세포활력（발광치：284296.7±16541.2），명현저우대조조적（848586.7±61404.4）；복사폭로1 Gy후，복사조세포내ROS수평（형광치：6750.0±103.5）고우대조조적（5710.7±56.2），소서BMMNCs극륭형성능력복사조위（626.7±51.3）극륭수/105세포，저우대조조적（986.7±100.7）극륭수/105세포；이복사가NAC조，여복사조비교소서BMMNCs세포활력증가지발광치352770.0±23466.1、세포내ROS수평하강지형광치5430.0±61.0、BMMNCs극륭형성능력증가지（773.3±49.3）극륭수/105세포。결론 NAC대복사폭로소서BMMNCs손상가능시통과강저세포내ROS수평체도보호작용。NAC가이작위후속실험연구적양성대조약。
Objective To observe the protective effect of N-acetyl-cysteine (NAC) on the injury of irradiation-in-duced bone marrow mononuclear cells (BMMNCs), and explore the possible mechanism. Methods There were 3 groups in the study:control group, irradiation group (doses of irradiation were 1 Gy and 4 Gy) and irradiation with NAC group (NAC was cocultured with BMMNCs half hour before irradiation). The 2×106/mL BMMNCs and the RPMI-1640 medium or 2×10-5 mol/L NAC were added into the 2 mL EP tubes according to the different requirement of groups. The tubes were then cul-tured in the 37℃CO2 incubator for 30 min and irradiated with 1 Gy and 4 Gy. The viability of BMMNCs was measured by bioluminescence. The level of reactive oxygen species (ROS) was measured by DCFH-DA, and the ability of colony-forming units was detected by CFU-GM. Results After 4 Gy irradiation exposure, the cell viability of BMMNCs was significantly lower in irradiation group (284 296.7±16 541.2) than that of control group (848 586.7±61 404.4). After 1 Gy irradiation expo-sure, the level of ROS was higher in irradiation group (6 750.0±103.5) than that of control group (5 710.7±56.2). The number of colony-forming units per 105 cells after irradiation exposure was (626.7±51.3), which was significantly lower than that of control group (986.7±100.7). Compared to irradiation group, the cell viability of BMMNCs increased to (352 770.0±23 466.1) in irradiation with NAC group. The level of ROS decreased to (5 430.0±61.0), and the number of colony-forming units per 105 cells increased to (773.3 ± 49.3). Conclusion NAC has protective effect on irradiation-induced injury in BMMNCs, which may be related with the decreased level of ROS. NAC can play the role of positive control for the following work.