CAJ | 학술논문

目的：探讨五味子乙素（SchB）对长波紫外线（UVA）诱导永生化人角质形成细胞（HaCat细胞）损伤后的保护作用及其可能的作用机制。方法用5 J/cm2的UVA照射HaCat细胞，给予不同浓度的SchB（0.1、0.01、0.001、0.0001μmol/L）处理UVA损伤后的HaCat细胞，检测细胞超氧化物歧化酶（SOD）及谷胱甘肽过氧化物酶（GSH-Px）的活性，乳酸脱氢酶（LDH）及NO含量。结果 UVA照射HaCat细胞后，SOD、GSH-Px活性降低，LDH及NO含量升高，给予不同浓度的SchB均能显著提高SOD、GSH-Px活性，降低LDH及NO含量，提高HaCat细胞的存活率，尤其以0.001μmol/L SchB的保护作用最强。结论0.001μmol/L SchB减轻UVA对HaCat细胞损伤的效果最佳。
목적：탐토오미자을소（SchB）대장파자외선（UVA）유도영생화인각질형성세포（HaCat세포）손상후적보호작용급기가능적작용궤제。방법용5 J/cm2적UVA조사HaCat세포，급여불동농도적SchB（0.1、0.01、0.001、0.0001μmol/L）처리UVA손상후적HaCat세포，검측세포초양화물기화매（SOD）급곡광감태과양화물매（GSH-Px）적활성，유산탈경매（LDH）급NO함량。결과 UVA조사HaCat세포후，SOD、GSH-Px활성강저，LDH급NO함량승고，급여불동농도적SchB균능현저제고SOD、GSH-Px활성，강저LDH급NO함량，제고HaCat세포적존활솔，우기이0.001μmol/L SchB적보호작용최강。결론0.001μmol/L SchB감경UVA대HaCat세포손상적효과최가。
Objective To explore the protective effect of schisandrin B (SchB) on the permanent damage of human keratinocytes (HaCat) induced by long wave ultraviolet (UVA),and its possible mechanism thereof. Methods After HaCat was treated by 5 J/cm2 UVA, different concentrations of SchB (0.1, 0.01, 0.001 and 0.000 1μmol/L) were used to treat HaCat cells. The levels of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) activity, lactate dehydrogenase (LDH) and NO content were detected. Results The levels of SOD and GSH-Px activity were decreased, and he levels of LDH and NO content were increased in HaCat cells after being treated by UVA. The different concentrations of SchB showed significant ef-fects on the increased levels of SOD, GSH-Px activity and decreased levels of LDH and NO, and improved the survival rate of HaCat cells. The 0.001 μmol/L SchB showed the strongest protective effect. Conclusion The 0.001 μmol/L SchB showed the best effect on the damage of HaCat cells induce the UVA.