CAJ | 학술논문

采用不同浓度NaCl溶液(0,50,150,250 mmol/L)处理萌发的垂穗披碱草幼苗,通过检测垂穗披碱草在盐胁迫逆境下生理生化的变化,并与老芒麦进行比较,研究其幼苗对盐胁迫的反馈机制,为垂穗披碱草在盐碱地的建植和恢复改良提供参考。结果表明：垂穗披碱草叶片可溶性糖质量分数和过氧化氢酶活性变化一致,随着盐浓度的增加呈现先上升后下降的趋势,50 mmol/L NaCl是其幼苗可溶性糖质量分数和过氧化氢酶活性的一个转折临界点；相对含水量、脯氨酸质量分数和超氧化物歧化酶活性变化一致,随着盐浓度的增加呈现上升趋势,分别在150,250 mmol/L盐浓度胁迫下,垂穗披碱草幼苗相对含水量、脯氨酸质量分数和超氧化物歧化酶活性显著高于其他材料的。方差分析结果表明盐胁迫对垂穗披碱草、老芒麦的生理指标影响显著。经相关分析筛选出对抗盐性影响显著的指标为相对含水量、可溶性糖质量分数、脯氨酸质量分数、超氧化物歧化酶、过氧化氢酶活性。经隶属函数法计算得出,垂穗披碱草具有一定的抗盐性,而且较老芒麦的强。
채용불동농도NaCl용액(0,50,150,250 mmol/L)처리맹발적수수피감초유묘,통과검측수수피감초재염협박역경하생리생화적변화,병여로망맥진행비교,연구기유묘대염협박적반궤궤제,위수수피감초재염감지적건식화회복개량제공삼고。결과표명：수수피감초협편가용성당질량분수화과양화경매활성변화일치,수착염농도적증가정현선상승후하강적추세,50 mmol/L NaCl시기유묘가용성당질량분수화과양화경매활성적일개전절림계점；상대함수량、포안산질량분수화초양화물기화매활성변화일치,수착염농도적증가정현상승추세,분별재150,250 mmol/L염농도협박하,수수피감초유묘상대함수량、포안산질량분수화초양화물기화매활성현저고우기타재료적。방차분석결과표명염협박대수수피감초、로망맥적생리지표영향현저。경상관분석사선출대항염성영향현저적지표위상대함수량、가용성당질량분수、포안산질량분수、초양화물기화매、과양화경매활성。경대속함수법계산득출,수수피감초구유일정적항염성,이차교로망맥적강。
Compared with those of Elymus sibiricus, the growth parameters of Elymus nutans at the early seedling stage were measured under four NaCl solutions with different concentrations (0, 50, 150, 250 mmol/L) and salt treatment was performed to explore and utilize the germplasm with high salt-tolerance and to provide physiological indicators for identifying salt-tolerance. It was found that the soluble sugar content and the activities of CAT were increasing at first and then declining with prolonged salt stress; a critical turning point occurred under the salt stress at 150 mmol/L, the relative water content, the proline content and the activities of SOD increased continually with NaCl stress concentration being increased, and accessions were higher than the others when the salt concentration was at 150 mmol/L and 250 mmol/L. Statistics analysis showed that the effects were different for E. nutans and E. sibiricus under salt stress. Correlation analysis indicated that the indices which had significant impact on salt resistance were the relative water content, soluble sugar content, proline content, and the activities of SOD and CAT. Calculated by the membership function, the salt-resistant ability of E. nutans was better than that of E. sibiricus.