CAJ | 학술논문

实验以枸杞种子为材料,培育成无菌苗后,取叶片和下胚轴进行诱导愈伤组织,在含有2,4-D的MS培养基上继代培养并诱导体细胞胚发生.通过从不同时期的胚性愈伤组织中剥离不同时期的体细胞胚,对它们进行形态学观察和发生频率统计,进而研究不同枸杞品种、2,4-D质量浓度、继代培养次数、不同部位和温度对枸杞体细胞胚发生的影响.结果表明：在含有0.2 mg/L 2,4-D的MS培养基上,继代3次的愈伤组织经诱导分化体细胞胚可达496个/g；同时还探讨了枸杞体细胞胚高频发生再生体系建立的关键影响因素,为体细胞胚发生机理的研究和应用提供依据.
실험이구기충자위재료,배육성무균묘후,취협편화하배축진행유도유상조직,재함유2,4-D적MS배양기상계대배양병유도체세포배발생.통과종불동시기적배성유상조직중박리불동시기적체세포배,대타문진행형태학관찰화발생빈솔통계,진이연구불동구기품충、2,4-D질량농도、계대배양차수、불동부위화온도대구기체세포배발생적영향.결과표명：재함유0.2 mg/L 2,4-D적MS배양기상,계대3차적유상조직경유도분화체세포배가체496개/g；동시환탐토료구기체세포배고빈발생재생체계건립적관건영향인소,위체세포배발생궤리적연구화응용제공의거.
Seeds of Lycium barbarum L. were taken as raw material to develop into sterile seedlings firstly. Cytyledon and hypocotyl of these sterile seedlings were employed to produce callus. And then somatic embryo-genesis was induced from the MS medium with 2, 4-D. By observing the shape and counting the number of the somatic embryos that were stripped from the embryonic callus in different periods, our study focused on factors might influence the rate of somatic embryogenesis of Lycium barbarum L. such as the breed of Lycium barbarum L., the concentration of 2, 4-D, the subculture times, explant and temperature. The results showed that the callus, passaged three times in the MS medium with 0.2 mg/L 2, 4-D, had the highest rate of embryogenesis. Each gram had up to 496 somatic embryos. The paper revealed the regulation mechanism of somatic embryo-genesis of Lycium barbarum L. and provides a basis for the research and application of somatic embryos.