CAJ | 학술논문

采用水蒸气蒸馏法提取海南产神秘果叶挥发油，利用气相色谱-质谱联用技术( GC-MS)分析挥发油的化学成分，并对挥发油的抑菌和抗肿瘤活性进行了评价。结果从挥发油中共分离出68个化合物，鉴定出44种化学成分，占挥发油总质量分数的92.14%。其中质量分数较高的组分为匙叶桉油烯醇(24.194%)，柠檬烯(15.805%)，邻苯二甲酸二异辛酯(12.402%)，邻苯二甲酸二丁酯(10.326%)，棕榈酸(4.865%)和芳樟醇(2.139%)。体外抗菌试验的结果表明，神秘果叶挥发油除了对绿脓杆菌没有明显的抑制作用，对其他7个试验菌株均具有较好的抑菌活性，最小抑菌浓度( MIC)范围为39.06~252.15 mg/L。抗肿瘤活性试验的结果表明，神秘果叶挥发油能够有效抑制人白血病细胞K562的增殖，半数抑制浓度(IC50)值为13.5 mg/L。
채용수증기증류법제취해남산신비과협휘발유，이용기상색보-질보련용기술( GC-MS)분석휘발유적화학성분，병대휘발유적억균화항종류활성진행료평개。결과종휘발유중공분리출68개화합물，감정출44충화학성분，점휘발유총질량분수적92.14%。기중질량분수교고적조분위시협안유희순(24.194%)，저몽희(15.805%)，린분이갑산이이신지(12.402%)，린분이갑산이정지(10.326%)，종려산(4.865%)화방장순(2.139%)。체외항균시험적결과표명，신비과협휘발유제료대록농간균몰유명현적억제작용，대기타7개시험균주균구유교호적억균활성，최소억균농도( MIC)범위위39.06~252.15 mg/L。항종류활성시험적결과표명，신비과협휘발유능구유효억제인백혈병세포K562적증식，반수억제농도(IC50)치위13.5 mg/L。
The aim of this study was to determine the chemical composition of Synsepalum dulcificum leaf essential oil obtained by steam distillation. The in vitro antibacterial activity and antitumoral activity of this essential oil was characterized. Sixty-eight compositions were separated. Among them 44 components were identified and represented 92. 14% of the total detected constituents. The major chemical compounds of leaf essential oil were spathulenol (24.194%), limonene ( 15. 805%), diisooctyl phthalate ( 12. 402%), dibutyl phthalate ( 10. 326%), palmitic acid (4.865%) and linalool (2. 139%). The result of the antimicrobial assay showed that the essential oil displayed varying degrees of antibacterial activity against all tested bacterial except for Pseudomonas aeruginosa, along with minimum inhibition concentration(MIC)values from 39. 06 to 252. 15 mg/L. In addition, the antitumoral activity using MTT assay of the leaf essential oil exhibited this oil was effective against human K562 cancer cell line in a dose dependent manner. Its inhibition concentration 50% (IC50) value was 13. 5 mg/L.