CAJ | 학술논문

以陕西长枣皮为原料,采用超声波减压法提取其中多酚,通过单因素试验及正交试验L9(34)考察了各因素间的交互作用,优化了超声波辅助减压提取枣皮多酚的条件。在此基础上,对比了常规浸提、超声浸提和超声波辅助减压提取三者对提取得率和提取时间的影响,并通过清除自由基能力( DPPH·)衡量了各种提取方法的优劣。结果表明：超声波辅助减压提取枣皮多酚的最佳工艺参数为乙醇体积分数50%,提取温度55℃,液料比30：1(mL：g),超声波功率240 W,提取时间10 min,枣皮多酚得率可达5.05%,此条件下提取2次,多酚得率为6.33%,提取率即达97.37%；对比热浸提和超声波浸提,超声波辅助减压提取在明显缩短提取时间的同时又能显著提高枣皮多酚得率；清除自由基能力( DPPH·)测试表明超声波辅助减压提取所得的多酚粗提物活性最高,清除率88.4%,该方法能够更好的保护枣皮多酚的生物活性。
이협서장조피위원료,채용초성파감압법제취기중다분,통과단인소시험급정교시험L9(34)고찰료각인소간적교호작용,우화료초성파보조감압제취조피다분적조건。재차기출상,대비료상규침제、초성침제화초성파보조감압제취삼자대제취득솔화제취시간적영향,병통과청제자유기능력( DPPH·)형량료각충제취방법적우렬。결과표명：초성파보조감압제취조피다분적최가공예삼수위을순체적분수50%,제취온도55℃,액료비30：1(mL：g),초성파공솔240 W,제취시간10 min,조피다분득솔가체5.05%,차조건하제취2차,다분득솔위6.33%,제취솔즉체97.37%；대비열침제화초성파침제,초성파보조감압제취재명현축단제취시간적동시우능현저제고조피다분득솔；청제자유기능력( DPPH·)측시표명초성파보조감압제취소득적다분조제물활성최고,청제솔88.4%,해방법능구경호적보호조피다분적생물활성。
In order to obtain maximum yield of polyphenols from jujube peel, ultrasound-assisted vacuum extraction ( UAVE) was chosen to extract the peel of Ziziphus jujube var. Changzao. The effects of various conditions were investigated by single factor experiments and orthogonal design L9 ( 34 ) . Based on the optimization condition, three extraction methods, i. e. , UAVE, ultrasound assist extraction(UAE) and conventional extraction(CE), were compared with both extraction time and polyphenols yield. To further evaluate UAVE, the radical scavenging activities ( DPPH·) was measured, too. Results showed that: the optimal conditions were 50 % aqueous ethanol as extraction solvent, temperature 55 ℃, solid/liquid ratio 1:30 ( g: mL ) , ultrasonic power 240 W, extraction time 10 min. Under this condition, the polyphenols yield could be 5. 05 %, and the extraction rate could reach 97. 37 % with duplicate extraction. UAVE has advantages such as high yield and time saving by comparison with CE and UAE. The polyphenols extracted by UAVE showed that higher antioxidant activities could be obtained than those by CE and UAE. It indicated that UAVE could protect the bioactive capacity of polyphenols from jujube peel.