CAJ | 학술논문

目的：建立黄白通气颗粒的质量控制标准。方法采用薄层色谱法（T LC法）对大黄进行定性鉴别；采用高效液相色谱法（HPLC法）对大黄素和大黄酚进行定量测定。结果 TLC能对大黄进行专属定性分析；大黄素在01．25～03．74μg范围内与峰面积呈良好的线性关系，r ＝09．999，平均回收率为979．％，RSD＝13．1％；大黄酚在01．50～04．51μg范围内与峰面积呈良好的线性关系，r ＝09．999，平均回收率为982．％，RSD＝13．5％。三批样品中大黄素、大黄酚总含量分别为156．7、157．4、158．8 m g/袋。结论所建立的方法稳定、准确，重现性好，可用于黄白通气颗粒的质量控制。
목적：건립황백통기과립적질량공제표준。방법채용박층색보법（T LC법）대대황진행정성감별；채용고효액상색보법（HPLC법）대대황소화대황분진행정량측정。결과 TLC능대대황진행전속정성분석；대황소재01．25～03．74μg범위내여봉면적정량호적선성관계，r ＝09．999，평균회수솔위979．％，RSD＝13．1％；대황분재01．50～04．51μg범위내여봉면적정량호적선성관계，r ＝09．999，평균회수솔위982．％，RSD＝13．5％。삼비양품중대황소、대황분총함량분별위156．7、157．4、158．8 m g/대。결론소건립적방법은정、준학，중현성호，가용우황백통기과립적질량공제。
Objective To establish the quality standard for Huangbai Tongqi Granules .Methods Rhubarb was identified by TLC ;Emodin and Chrysophanol were determined by HPLC .Results Rhubarb could be detected by TLC ;Emodin showed a good linear relationship at a range of 0 1.25~0 3.74 μg ,r =0 9.99 9 . The average recovery was 97 9.% ,and RSD was 1 3.1% ;Chrysophanol showed a good linear relationship at a range of 0 1.50~0 4.51 μg ,r =0 9.99 9 .The average recovery was 98 2.% ,and RSD was 1 3.5% ;Conclu-sion T he established methods are stable ,accurate and reproducible .It can be used for the quality control of Huangbai Tongqi Granules .