CAJ | 학술논문

为分离出可用作猪用微生态制剂的肠道益生菌株，本研究选择锦州市凌海大业乡生态猪场为试验采样点，采集10日龄左右健康仔猪肠道粪样12份。利用MRS琼脂培养基分离培养初筛目标分离菌株的形态特征和培养特性，再通过生理生化鉴定法和16SrRNA基因分子鉴定法，最终确定1株分离菌为屎肠球菌，并命名为LN/EF1312株，为后续肠道益生球菌的益生功能研究奠定了物质基础。
위분리출가용작저용미생태제제적장도익생균주，본연구선택금주시릉해대업향생태저장위시험채양점，채집10일령좌우건강자저장도분양12빈。이용MRS경지배양기분리배양초사목표분리균주적형태특정화배양특성，재통과생리생화감정법화16SrRNA기인분자감정법，최종학정1주분리균위시장구균，병명명위LN/EF1312주，위후속장도익생구균적익생공능연구전정료물질기출。
In order to isolate the intestinal live strains which can be used to develop probiot-ics additive for pig, a ecological pig farm in Daye township of Linghai in Jinzhou city were se-lected for gathering intestinal fecal samples of healthy piglets with about 10-day age. The total 12 samples were isolated cultured using MRS agar medium at the beginning, then isolated strain clones with the classic culture features and morphological characteristics, were subsequently identified through the physiological and biochemical assay method combining with 16S rRNA gene mo-lecular identification method, finally a confirmed isolated strain of enterococcus faecium was named LN/EF1312 strain. This result provide foundation for the research on the function of live intestinal enterococcus.