CAJ | 학술논문

以原油作为惟一碳源，对长庆油田石油污染土壤中石油降解菌进行富集培养并筛选出高效石油降解菌，研究石油污染土壤微生物特性及石油降解菌降解性能．结果表明，长庆油田受污染土壤石油降解菌为杆状或球状，既有革兰氏阴性菌，又有革兰氏阳性菌；在好氧条件下，石油烃降解优势菌株a，b，c，d有较强的降解能力，分别属于邻单胞菌属，芽孢杆菌属和动胶菌属；a和b在初始p H为9时降解率最高分别为69.81％和71.41％；c和d在初始p H为7时降油率最高分别为66.94％和65.76％．
이원유작위유일탄원，대장경유전석유오염토양중석유강해균진행부집배양병사선출고효석유강해균，연구석유오염토양미생물특성급석유강해균강해성능．결과표명，장경유전수오염토양석유강해균위간상혹구상，기유혁란씨음성균，우유혁란씨양성균；재호양조건하，석유경강해우세균주a，b，c，d유교강적강해능력，분별속우린단포균속，아포간균속화동효균속；a화b재초시p H위9시강해솔최고분별위69.81％화71.41％；c화d재초시p H위7시강유솔최고분별위66.94％화65.76％．
By using crude oil as the sole carbon source ,the petroleum degrading bacteria in polluted soil of Changqing oilfield was concentrated with enrichment culture and highly efficient petroleum degrading strains were screened .And the performance characteristics and petroleum degradability of these strains were explored .The results showed that Changqing oilfield contaminated soil petroleum degrading bacte-ria were bacillus or coccus ,either gram negative or gram positive bacteria .Under aerobic conditions , hydrocarbon degrading strains a ,b ,c ,d had strong degradation capacity .Among them ,a and b be-longed to Plesiomonas ,c belonged to Bacillus ,d belonged to Zoogloea .A and b had the highest degra-dation rate of 69 .81% and 71 .41% at the initial pH of 9 .C and d had the highest oil degradation rate of 66 .94% and 65 .76% at the initial pH of 7 .