食品与发酵科技
食品與髮酵科技
식품여발효과기
SICHUAN FOOD AND FERMENTATION
2013年
6期
68-72
,共5页
冯治平%赵斌%黄治国%邓杰%刘燕梅%祝云飞%王艳丽
馮治平%趙斌%黃治國%鄧傑%劉燕梅%祝雲飛%王豔麗
풍치평%조빈%황치국%산걸%류연매%축운비%왕염려
白酒酒醅%古菌%PCR-SSCP%16S rDNA
白酒酒醅%古菌%PCR-SSCP%16S rDNA
백주주배%고균%PCR-SSCP%16S rDNA
liquor fermented grains%archaea%PCR-SSCP%16S rDNA
该试验采用PCR-SSCP技术研究了浓香型白酒酒醅发酵过程中古菌群落的变化规律,结果发现:所有酒醅样品均出现5~10条较清晰的条带,其中第2、4、9号条带在所有样品中均有检出,且优势度较高;所有样品古菌群落生物多样性指数都在1.53~2.01之间,在发酵过程中波动较小;酒醅古菌SSCP 图谱相似性指数较高,表明古菌群落在发酵过程中的变化较小。
該試驗採用PCR-SSCP技術研究瞭濃香型白酒酒醅髮酵過程中古菌群落的變化規律,結果髮現:所有酒醅樣品均齣現5~10條較清晰的條帶,其中第2、4、9號條帶在所有樣品中均有檢齣,且優勢度較高;所有樣品古菌群落生物多樣性指數都在1.53~2.01之間,在髮酵過程中波動較小;酒醅古菌SSCP 圖譜相似性指數較高,錶明古菌群落在髮酵過程中的變化較小。
해시험채용PCR-SSCP기술연구료농향형백주주배발효과정중고균군락적변화규률,결과발현:소유주배양품균출현5~10조교청석적조대,기중제2、4、9호조대재소유양품중균유검출,차우세도교고;소유양품고균군락생물다양성지수도재1.53~2.01지간,재발효과정중파동교소;주배고균SSCP 도보상사성지수교고,표명고균군락재발효과정중적변화교소。
The experiment researched the changing laws of eukaryotic microbes community in fermentation process of fermented grains of luzhou-flavor liquor by PCR-SSCP technique. The results were as follows: clear bands from 5 to 10 were showed clearly in all grains samples, NO.2、4、9 bands were detected in all samples and they had a high dominant degree. The diversity index of all samples kept between 1.53 and 2.01, the diversity index of samples generally showed small fluctuations during the fermentation process. The similarity indexs of PCR-SSCP pattern of samples were high, which means archaea community had small changes in the fermentation process.