CAJ | 학술논문

从成品茶中快速、高效地提取高质量的基因组DNA ，是对成品茶进行分子鉴别的前提。利用简化CTAB法和改进CTAB法，提取茶树品种金牡丹鲜叶及其加工而成的红茶、绿茶、乌龙茶基因组DNA ，并进行PCR验证，结果表明：2种方法均能提取成品茶基因组DNA ；与鲜叶材料相比，虽然成品茶基因组DNA均呈现不同程度的降解现象，但主带清晰；2种方法所提取的基因组DNA质量无显著差异，但简化CTAB法由于操作简单，更适宜实际应用；提取的成品茶基因组DNA均可以用来进行PAPD以及SSR分析。
종성품다중쾌속、고효지제취고질량적기인조DNA ，시대성품다진행분자감별적전제。이용간화CTAB법화개진CTAB법，제취다수품충금모단선협급기가공이성적홍다、록다、오룡다기인조DNA ，병진행PCR험증，결과표명：2충방법균능제취성품다기인조DNA ；여선협재료상비，수연성품다기인조DNA균정현불동정도적강해현상，단주대청석；2충방법소제취적기인조DNA질량무현저차이，단간화CTAB법유우조작간단，경괄의실제응용；제취적성품다기인조DNA균가이용래진행PAPD이급SSR분석。
Rapid and efficient extraction of genomic DNA from tea products is essential for molecular identification . The genomic DNAs of the fresh tea leaves of the tea cultivars ,Jin Mu-dan ,as well as the black ,green and oolong teas made from them were extracted by means of the simplified and the improved CTAB methods . The results were verified by means of PCR . It was found that both of the methods performed satisfactorily for the extraction . Compared with the fresh leaves ,the processed teas showed different degrees of degradation ,but the main band was clear . There appeared no significant difference on the quality of the extracted genomic DNAs between the two methods . The data obtained by either method on the teas could be used for the PAPD and SSR analyses without concerns . However ,the simplified CTAB method appeared simpler to operate than the modified method .