实用癌症杂志
實用癌癥雜誌
실용암증잡지
THE PRACTICAL JOURNAL OF CANCER
2014年
4期
379-380
,共2页
胃癌%核苷酸切除修复交叉互补基因1%甲基化%甲基化特异性聚合酶链反应
胃癌%覈苷痠切除脩複交扠互補基因1%甲基化%甲基化特異性聚閤酶鏈反應
위암%핵감산절제수복교차호보기인1%갑기화%갑기화특이성취합매련반응
Gastric cancer%Excision repair cross-complementing gene 1 ( ERCC1 )%Methylation%Methylation specific polymerase chain reaction
目的:检测胃癌组织、相应癌旁组织及正常胃组织中ERCC1基因甲基化状态。方法采用甲基化特异性PCR(MSP)技术检测ERCC1基因甲基化。结果胃癌组织中ERCC1基因完全甲基化率为46.7%(14/30),部分甲基化率为30.0%(9/30),总甲基化率为76.7%(23/30),显著高于相应的癌旁组织中该基因的甲基化率13.3%(4/30)。10例正常胃组织中该基因未发生甲基化。结论胃癌组织中存在高比例的ERCC1基因甲基化。
目的:檢測胃癌組織、相應癌徬組織及正常胃組織中ERCC1基因甲基化狀態。方法採用甲基化特異性PCR(MSP)技術檢測ERCC1基因甲基化。結果胃癌組織中ERCC1基因完全甲基化率為46.7%(14/30),部分甲基化率為30.0%(9/30),總甲基化率為76.7%(23/30),顯著高于相應的癌徬組織中該基因的甲基化率13.3%(4/30)。10例正常胃組織中該基因未髮生甲基化。結論胃癌組織中存在高比例的ERCC1基因甲基化。
목적:검측위암조직、상응암방조직급정상위조직중ERCC1기인갑기화상태。방법채용갑기화특이성PCR(MSP)기술검측ERCC1기인갑기화。결과위암조직중ERCC1기인완전갑기화솔위46.7%(14/30),부분갑기화솔위30.0%(9/30),총갑기화솔위76.7%(23/30),현저고우상응적암방조직중해기인적갑기화솔13.3%(4/30)。10례정상위조직중해기인미발생갑기화。결론위암조직중존재고비례적ERCC1기인갑기화。
Objective To detect the methylation status of ERCC 1 gene in gastric cancer tissues ,paired adjacent cancer-ous tissues and normal gastric tissues .Methods Methylation-specific PCR ( MSP) was used to detect the methylation of ERCC 1 gene.Results The complete methylation rate of ERCC1 gene in gastric cancer tissues was 46.7%(14/30),the partial methyla-tion rate was 30.0%(9/30),and the total methylation rate was 76.7%(23/30),which was significantly higher than the methy-lation rate in adjacent cancerous tissues (13.3%,4/30).There was no methylation in 10 cases of normal gastric tissues .Conclu-sion There is a high percentage of ERCC 1 gene methylation in gastric cancer tissues .