CAJ | 학술논문

目的：研究CD31基因的单核苷酸多态性和体内血清含量与原发性肝癌的关联性。方法选取160例肝癌患者和160例健康体检者作为观察对象，采用PCR-RFLP技术对CD31基因Leu125Val、Asn563Ser和Gly670Arg单核苷酸多态性进行基因分型，并且采用酶联免疫吸附试验检测血清CD31含量。结果肝癌患者和健康体检者的CD31基因Asn563Ser和Gly670Arg多态性差异不明显（χ2＝0．387，P＞0．05），而Leu125Val 多态性差异显著（χ2＝10．875，P＜0．05）。Val等位基因携带者患肝癌的风险是Leu等位基因的1．457倍（OR＝1．457，95％CI：1．187～2．186，P＝0．001）；携带Val等位基因的原发性肝癌患者的血清CD31含量比没有携带的患者要高很多（χ2＝10．534，P＜0．05）。结合基因型分析表明，CD31基因Leu125Val、Asn563Ser和Gly670Arg单核苷酸多态性具有明显的连锁不平衡，同健康体检者相比， Val-Ser-Arg单倍型携带者罹患肝癌的风险较高（OR＝1．487，95％CI：1．092-2．038，P＝0．011）。结论 CD31基因的Leu125Val多态性及Val-Ser-Arg单倍型与原发性肝癌具有一定的关联，其中Val等位基因有极大的可能是原发性肝癌的遗传易感基因，携带Val基因型的患者可能会由于CD31基因在体内高度表达而增加罹患肝癌的可能性。
목적：연구CD31기인적단핵감산다태성화체내혈청함량여원발성간암적관련성。방법선취160례간암환자화160례건강체검자작위관찰대상，채용PCR-RFLP기술대CD31기인Leu125Val、Asn563Ser화Gly670Arg단핵감산다태성진행기인분형，병차채용매련면역흡부시험검측혈청CD31함량。결과간암환자화건강체검자적CD31기인Asn563Ser화Gly670Arg다태성차이불명현（χ2＝0．387，P＞0．05），이Leu125Val 다태성차이현저（χ2＝10．875，P＜0．05）。Val등위기인휴대자환간암적풍험시Leu등위기인적1．457배（OR＝1．457，95％CI：1．187～2．186，P＝0．001）；휴대Val등위기인적원발성간암환자적혈청CD31함량비몰유휴대적환자요고흔다（χ2＝10．534，P＜0．05）。결합기인형분석표명，CD31기인Leu125Val、Asn563Ser화Gly670Arg단핵감산다태성구유명현적련쇄불평형，동건강체검자상비， Val-Ser-Arg단배형휴대자리환간암적풍험교고（OR＝1．487，95％CI：1．092-2．038，P＝0．011）。결론 CD31기인적Leu125Val다태성급Val-Ser-Arg단배형여원발성간암구유일정적관련，기중Val등위기인유겁대적가능시원발성간암적유전역감기인，휴대Val기인형적환자가능회유우CD31기인재체내고도표체이증가리환간암적가능성。
Objective To investigate the association of single nucleotide polymorphisms ( SNPs) of CD31 and serum level with hepatocellular carcinoma(HCC).Methods Three single nucleotide polymorphisms Leu125Val,Asn563Ser and Gly670Arg of CD31 were analyzed using polymerase chain reaction-restriction fragment length polymorphism ( PCR-RFLP ) , and the serum level of CD31 was determined by enzyme-linked immunosorbent assay ( ELISA) in 160 HCC patients and 160 healthy patients .Re-sults The CD31 gene Asn563Ser and Gly670Arg polymorphisms had no significant difference between the HCC group and the control group(χ2 =0.387,P>0.05),but the CD31 gene Leu125Val polymorphism of the 2 groups had significant difference (χ2=10.875,P<0.05).The relative risk of HCC of Val allele was 1.457 times that of the Leu allele carriers(OR=1.457,95%CI:1.187~2.186,P=0.001);Serum level of CD31 of Val allele carriers was significantly higher than that of no carriers (χ2 =10.534,P<0.05).Genotype analysis showed that CD31 gene Leu125Val,Asn563Ser and Gly670Arg polymorphisms had strong linkage disequilibrium ,the Val-Ser-Arg haplotype was associated with a high risk of HCC as compared with the healthy patients (OR=1.487,95%CI:1.092-2.038,P=0.011).Conclusion CD31 gene Leu125Val polymorphism and its Val-Ser-Arg haplo-type are associated with HCC ,and Val allele is very likely an important genetic susceptibility gene for HCC .CD31 Val allele carri-ers may subject to higher risk of HCC with enhanced CD 31 expression .