CAJ | 학술논문

2011和2012年福建省各养殖区引进的美洲鳗鲡（ Anguilla Rostrata）和双色鳗鲡（ Anguilla bicolor bicolor）在玻璃鳗培育阶段普遍发生“红肝病”，导致了较高的发病率和死亡率，给养殖生产造成严重的危害。本文应用透射电镜（ TEM）对各养殖区患“红肝病”的美洲鳗鲡和双色鳗鲡进行超微病理研究。结果显示：在患病美洲鳗鲡鳃组织的肌肉细胞间质中的纤维母细胞查见胞核内许多排列无序的疱疹病毒核衣壳，多数呈空心双环状，直径约80 nm～100 nm，少数核心致密呈线点状或蝌蚪状。在患病双色鳗鲡鳃上皮细胞核旁胞浆内可查见个别成熟病毒粒子。根据所检出病毒的形态和形态发生学特征可以确定该病毒为疱疹病毒科（ Herpesviridae）的成员。另外，在病鳗肾细胞的细胞突起之间和细胞外可查见许多大小不等、形态多样，呈杆状、球形、哑铃形或橄榄形，有双层脂质外膜，无细胞壁的支原体（ pleuro-pneumonia-like organism，PPLO）。本研究在国内首次应用TEM显微镜意外地在自然感染的养殖鳗鲡组织细胞中检出疱疹病毒和支原体，为鳗鲡“红肝病”的防控提供了有价值的科学依据。
2011화2012년복건성각양식구인진적미주만려（ Anguilla Rostrata）화쌍색만려（ Anguilla bicolor bicolor）재파리만배육계단보편발생“홍간병”，도치료교고적발병솔화사망솔，급양식생산조성엄중적위해。본문응용투사전경（ TEM）대각양식구환“홍간병”적미주만려화쌍색만려진행초미병리연구。결과현시：재환병미주만려새조직적기육세포간질중적섬유모세포사견포핵내허다배렬무서적포진병독핵의각，다수정공심쌍배상，직경약80 nm～100 nm，소수핵심치밀정선점상혹과두상。재환병쌍색만려새상피세포핵방포장내가사견개별성숙병독입자。근거소검출병독적형태화형태발생학특정가이학정해병독위포진병독과（ Herpesviridae）적성원。령외，재병만신세포적세포돌기지간화세포외가사견허다대소불등、형태다양，정간상、구형、아령형혹감람형，유쌍층지질외막，무세포벽적지원체（ pleuro-pneumonia-like organism，PPLO）。본연구재국내수차응용TEM현미경의외지재자연감염적양식만려조직세포중검출포진병독화지원체，위만려“홍간병”적방공제공료유개치적과학의거。
During summer and autumn of 2011and 2012 in Fujian,China,the“red liver disease” with high mortality wide spread over the introduced cultured eels of Anguilla. Rostrata and Anguilla. bicolor bicolor in the glass eel stage and caused serious damage to eel culture. The ultrapathological study of the diseased ells was performed. Under transmission electron microscope, coinfection of herpesvirus and PPLO were detected expectedly. Most of the nucleocapsid of herpesvirus in nucleus of gill fibroblast of infected eel ( A. Rostrata) were round in shape,double-shell with an electron-lucent core,about 80 nm to 100 nm in diameter, and only a few of them with a dense core in the shape of line dot or tadpole. In the cytoplasm of gill epithelial cells of Anguilla bicolor, a few number of virion were noticed. The morphological and morphogenetic characteristics of the virus detected from the infected cultured eels are compatible with the morphology of the family of Herpesviridae. PPLO with polymorphism and variety in size were detected in the ultrasection of the kidney cells of infected cultured eel. The natural detection of coinfection herpesvirus and PPLO in the cultured eels by using electron microscope is the first time in china which provided valuable scientific basis for prevention and control of“red liver disease” in cultured eels.