中国肿瘤临床
中國腫瘤臨床
중국종류림상
CHINESE JOURNAL OF CLINICAL ONCOLOGY
2014年
7期
449-452
,共4页
张松%田向永%袁孝伟%柯少瑞%孙志艳%莫赛军
張鬆%田嚮永%袁孝偉%柯少瑞%孫誌豔%莫賽軍
장송%전향영%원효위%가소서%손지염%막새군
Caveolin-1%多形性腺瘤%腮腺%手术切缘
Caveolin-1%多形性腺瘤%腮腺%手術切緣
Caveolin-1%다형성선류%시선%수술절연
Caveolin-1%pleomorphic adenoma%parotid gland%surgical margins
目的：探讨窖蛋白-1（Caveolin-1，Cav-1）在腮腺多形性腺瘤（pleomorphic adenoma，PA）中的表达及临床意义。方法：采用免疫组织化学法检测了9例正常腮腺、33例原发性PA距离瘤体中心（0 cm）及瘤旁组织中（分别距瘤体中心0.5、1.0、1.5和2.0 cm）Cav-1的表达，并进行统计学分析。结果：Cav-1在所有正常腮腺样本（100%）均有表达，其主要在导管系统细胞质和细胞膜中表达；除6例距瘤体中心0.5 cm和1例距瘤体中心1.0 cm的样本外，其余瘤旁样本均有Cav-1表达，其表达模式基本上与正常腮腺类似；在PA中，有10例样本（30.30%）表达Cav-1，且在细胞核中显著表达。Cav-1在多形性腺瘤肿瘤组织中的表达最低，并随着与瘤体中心距离的远近，其表达量逐渐升高。Cav-1的表达在瘤体与瘤旁组织间均存在明显差异（P<0.05），但瘤旁组织1.0、1.5和2.0 cm间Cav-1表达无显著性差异（P>0.05）。结论：在腮腺多形性腺瘤发生过程中，Cav-1中表达下调并出现了从细胞质、细胞膜到细胞核的转位现象。上述结果表明Cav-1在多形性腺瘤的发生发展中可能起着重要作用，其表达水平的变化可作为判定手术安全边缘的科学依据之一。
目的：探討窖蛋白-1（Caveolin-1，Cav-1）在腮腺多形性腺瘤（pleomorphic adenoma，PA）中的錶達及臨床意義。方法：採用免疫組織化學法檢測瞭9例正常腮腺、33例原髮性PA距離瘤體中心（0 cm）及瘤徬組織中（分彆距瘤體中心0.5、1.0、1.5和2.0 cm）Cav-1的錶達，併進行統計學分析。結果：Cav-1在所有正常腮腺樣本（100%）均有錶達，其主要在導管繫統細胞質和細胞膜中錶達；除6例距瘤體中心0.5 cm和1例距瘤體中心1.0 cm的樣本外，其餘瘤徬樣本均有Cav-1錶達，其錶達模式基本上與正常腮腺類似；在PA中，有10例樣本（30.30%）錶達Cav-1，且在細胞覈中顯著錶達。Cav-1在多形性腺瘤腫瘤組織中的錶達最低，併隨著與瘤體中心距離的遠近，其錶達量逐漸升高。Cav-1的錶達在瘤體與瘤徬組織間均存在明顯差異（P<0.05），但瘤徬組織1.0、1.5和2.0 cm間Cav-1錶達無顯著性差異（P>0.05）。結論：在腮腺多形性腺瘤髮生過程中，Cav-1中錶達下調併齣現瞭從細胞質、細胞膜到細胞覈的轉位現象。上述結果錶明Cav-1在多形性腺瘤的髮生髮展中可能起著重要作用，其錶達水平的變化可作為判定手術安全邊緣的科學依據之一。
목적：탐토교단백-1（Caveolin-1，Cav-1）재시선다형성선류（pleomorphic adenoma，PA）중적표체급림상의의。방법：채용면역조직화학법검측료9례정상시선、33례원발성PA거리류체중심（0 cm）급류방조직중（분별거류체중심0.5、1.0、1.5화2.0 cm）Cav-1적표체，병진행통계학분석。결과：Cav-1재소유정상시선양본（100%）균유표체，기주요재도관계통세포질화세포막중표체；제6례거류체중심0.5 cm화1례거류체중심1.0 cm적양본외，기여류방양본균유Cav-1표체，기표체모식기본상여정상시선유사；재PA중，유10례양본（30.30%）표체Cav-1，차재세포핵중현저표체。Cav-1재다형성선류종류조직중적표체최저，병수착여류체중심거리적원근，기표체량축점승고。Cav-1적표체재류체여류방조직간균존재명현차이（P<0.05），단류방조직1.0、1.5화2.0 cm간Cav-1표체무현저성차이（P>0.05）。결론：재시선다형성선류발생과정중，Cav-1중표체하조병출현료종세포질、세포막도세포핵적전위현상。상술결과표명Cav-1재다형성선류적발생발전중가능기착중요작용，기표체수평적변화가작위판정수술안전변연적과학의거지일。
Objective:This study was conducted to explore the expression and clinical significance of caveolin-1 (Cav-1) in pleo-morphic adenoma and paraneoplastic tissues of the parotid gland. Methods:Immunohistochemistry was used to detect Cav-1 expres-sion in normal parotid glands, pleomorphic adenoma (0 cm), and paraneoplastic tissues (0.5, 1.0, 1.5, and 2.0 cm from the pleomorphic adenoma). Results: Cav-1 was expressed in all normal parotid glands and was mainly expressed in the cytoplasm and plasma mem-brane of duct systems. Except for six samples 0.5 cm away and one sample 1.0 cm away from the pleomorphic adenoma, Cav-1 was ex-pressed in other adjacent normal tissues, and the expression pattern of Cav-1 was similar to that in normal tissues. In pleomorphic ade-noma, Cav-1 only existed in seven samples (accounting for 30.30%) and was prominently expressed in the nucleus. The expression lev-el of Cav-1 was lowest in pleomorphic adenoma and progressively increased with increasing distance from the tumor. A difference in Cav-1 expression was detected between pleomorphic adenoma (0 cm) and paraneoplastic tissues (0.5, 1.0, 1.5, and 2.0 cm from the tu-mor, P<0.05). However, no statistical significance existed between paraneoplastic tissues at 1.0, 1.5, and 2.0 cm from the tumor (P>0.05). Conclusion:During the development of pleomorphic adenoma, the expression level of Cav-1 progressively decreased, and trans-location of Cav-1 expression from cytoplasm and plasma membrane to nucleus occurred. Cav-1 may have an important role in the prog-ress of pleomorphic adenoma, and the variation in the expression level of Cav-1 may be used as a scientific evidence to determine the safe surgical margin of pleomorphic adenoma.
