CAJ | 학술논문

目的：研究银杏叶提取物（EGB）对 SW480结肠癌细胞生长增殖的影响。方法体外培养人结肠癌SW480细胞后，给予不同浓度（100,200,300,400,500mg·L-1）银杏叶提取物干预后，采用 CCK-8检测人结肠癌 SW480细胞的增殖抑制率，选择最佳作用浓度；随后，在此作用浓度下观察 EGB 作用不同时间对人结肠癌SW480细胞增殖抑制率的影响，并通过 Westernblot 检测人结肠癌 SW480细胞内 Ki67和 PCNA 的表达情况。结果银杏叶提取物可抑制结肠癌 SW480细胞的增殖，且随着作用浓度增加和作用时间的延长其抑制作用逐渐增强；300mg·L-1银杏叶提取物对结肠癌 SW480细胞增殖的抑制效果最明显。银杏叶提取物干预的结肠癌SW480细胞内 Ki67和 PCNA 的表达均明显下调。结论银杏叶提取物可呈浓度和时间依赖性地抑制结肠癌SW480细胞的增殖。
목적：연구은행협제취물（EGB）대 SW480결장암세포생장증식적영향。방법체외배양인결장암SW480세포후，급여불동농도（100,200,300,400,500mg·L-1）은행협제취물간예후，채용 CCK-8검측인결장암 SW480세포적증식억제솔，선택최가작용농도；수후，재차작용농도하관찰 EGB 작용불동시간대인결장암SW480세포증식억제솔적영향，병통과 Westernblot 검측인결장암 SW480세포내 Ki67화 PCNA 적표체정황。결과은행협제취물가억제결장암 SW480세포적증식，차수착작용농도증가화작용시간적연장기억제작용축점증강；300mg·L-1은행협제취물대결장암 SW480세포증식적억제효과최명현。은행협제취물간예적결장암SW480세포내 Ki67화 PCNA 적표체균명현하조。결론은행협제취물가정농도화시간의뢰성지억제결장암SW480세포적증식。
Objective To investigate the effects of oral ginkgo biloba extract (EGB) on the proliferation of SW480 colon cancer cell lines. Methods SW480 colon cancer cell lines were cultured in vitro. CCK-8 assay was used to measure the in-hibition rate of SW480 proliferation after treated by 100, 200, 300, 400 and 500 mg·L-1 EGB. Selected out the optimize con-centration of EGB and observed its influencing difference when treating the SW480 cell for different time. The expression of proliferation related indicators Ki67 and PCNA was detected using western blot. Results EGB inhibited the proliferation of SW480 cells with the increase of EGB concentration and treatment time. Both the expressions of Ki67 and PCNA decreased after GBM intervention. Conclusion EGB significantly suppressed the proliferation of colon cancer cell lines SW480 in a concentration and time dependent manner.