CAJ | 학술논문

目的：探讨内源性硫化氢（hydrogensulfide，H2S）是否参与了耐热肝癌细胞 HepG-2/thermotolerance （HepG-2/tt）的耐热。方法通过 PI 染色流式细胞仪检测和比较 HepG-2细胞与 HepG-2/tt 细胞耐热后的细胞凋亡率，用亚甲基蓝显色分光光度计法检测和比较 HepG-2细胞与 HepG-2/tt 细胞耐热后 H2S 生成量和H2S 合酶活性的差异。结果43℃环境培养24h 后，耐热肝癌细胞 HepG-2/tt 的细胞凋亡率较普通肝癌细胞HepG-2明显下降（P<0.01）；且 HepG-2/tt 细胞中 H2S 含量和 H2S 合酶活性均较普通 HepG-2细胞明显增加（P<0.01）。结论HepG-2/tt 较 HepG-2细胞具有热耐受性，并且其内源性硫化氢合酶活性和硫化氢生成量均有提高。
목적：탐토내원성류화경（hydrogensulfide，H2S）시부삼여료내열간암세포 HepG-2/thermotolerance （HepG-2/tt）적내열。방법통과 PI 염색류식세포의검측화비교 HepG-2세포여 HepG-2/tt 세포내열후적세포조망솔，용아갑기람현색분광광도계법검측화비교 HepG-2세포여 HepG-2/tt 세포내열후 H2S 생성량화H2S 합매활성적차이。결과43℃배경배양24h 후，내열간암세포 HepG-2/tt 적세포조망솔교보통간암세포HepG-2명현하강（P<0.01）；차 HepG-2/tt 세포중 H2S 함량화 H2S 합매활성균교보통 HepG-2세포명현증가（P<0.01）。결론HepG-2/tt 교 HepG-2세포구유열내수성，병차기내원성류화경합매활성화류화경생성량균유제고。
Objective To investigate whether endogenous hydrogen sulfide (H2S) involved in thermotolerance of HepG-2 (HepG-2/tt). Methods Flow cytometry with PI staining was used to measure the apoptosis of HepG-2 and HepG-2/tt after thermotolerance; Methylene blue Colorimetry was used to detect the production of hydrogen sulfide and the activity of en-dogenous synthases of HepG-2 and HepG-2/tt. Results After exposed in 43℃ for 24 hours, the apoptosis rate of HepG-2/tt cells was decreased significantly when compared with normal HepG-2 cells (P<0.01). In addition, the production of Hy-drogen sulfide and the activity of endogenous synthases of HepG-2/tt were significantly increased (P<0.01). Conclusions HepG-2/tt cells had greater heat tolerance than normal HepG-2 cells, and much more endogenous synthases activity and hydrogen sulfide.