中国急救复苏与灾害医学杂志
中國急救複囌與災害醫學雜誌
중국급구복소여재해의학잡지
CHINA JOURNAL OF EMERGENCY RESUSCITATION AND DISASTER MEDICINE
2014年
1期
33-35,42
,共4页
刘绍辉%何明丰%张英俭%陈景利%毛荣军
劉紹輝%何明豐%張英儉%陳景利%毛榮軍
류소휘%하명봉%장영검%진경리%모영군
参附注射液%心肺复苏%超微结构%心肌组织%脑海马组织%新西兰兔
參附註射液%心肺複囌%超微結構%心肌組織%腦海馬組織%新西蘭兔
삼부주사액%심폐복소%초미결구%심기조직%뇌해마조직%신서란토
Shenfu injection%Cardiac arrest%Cardiopulmonary resuscitation%Ultrastructure%Myocardium%Hippocampus%Rabbit
目的：研究参附注射液对新西兰兔心搏骤停复苏后心肌组织超微结构的影响，评价其对复苏后心肌组织的保护作用。方法采用窒息法制作新西兰兔心肺复苏模型，随机分为参附注射液组（A组）、正常复苏组（B组）、假手术模型组（C组）。夹闭气管后8 min（心搏骤停后约3 min）开始心肺复苏，自主循环恢复后 A 组给予静脉参附注射液2.1ml/kg（生理盐水稀释至5 ml），B 组给予静脉注射注射生理盐水5 ml，每隔30 min缓慢静脉注射1次，连续3次；C组静脉仅进行插管等手术操作，不进行呼吸心搏骤停处理和心肺复苏操作。观察复苏后三组动物MAP等变化。自主循环恢复后8 h处死动物，取心肌组织和脑海马CA区组织，光镜观察心肌和脑组织的变化、电镜观察超微结构改变。结果在HE染色光镜观察下心肌组织和脑组织的A组和B组之间变化的差异不大，但在电镜观察下则各组间显示差异较大，A组的损害相对较少。结论心肺复苏时应用参附注射液可以减轻心肌和脑组织超微结构损害，对复苏后心肌和脑组织有一定的保护作用。
目的：研究參附註射液對新西蘭兔心搏驟停複囌後心肌組織超微結構的影響，評價其對複囌後心肌組織的保護作用。方法採用窒息法製作新西蘭兔心肺複囌模型，隨機分為參附註射液組（A組）、正常複囌組（B組）、假手術模型組（C組）。夾閉氣管後8 min（心搏驟停後約3 min）開始心肺複囌，自主循環恢複後 A 組給予靜脈參附註射液2.1ml/kg（生理鹽水稀釋至5 ml），B 組給予靜脈註射註射生理鹽水5 ml，每隔30 min緩慢靜脈註射1次，連續3次；C組靜脈僅進行插管等手術操作，不進行呼吸心搏驟停處理和心肺複囌操作。觀察複囌後三組動物MAP等變化。自主循環恢複後8 h處死動物，取心肌組織和腦海馬CA區組織，光鏡觀察心肌和腦組織的變化、電鏡觀察超微結構改變。結果在HE染色光鏡觀察下心肌組織和腦組織的A組和B組之間變化的差異不大，但在電鏡觀察下則各組間顯示差異較大，A組的損害相對較少。結論心肺複囌時應用參附註射液可以減輕心肌和腦組織超微結構損害，對複囌後心肌和腦組織有一定的保護作用。
목적：연구삼부주사액대신서란토심박취정복소후심기조직초미결구적영향，평개기대복소후심기조직적보호작용。방법채용질식법제작신서란토심폐복소모형，수궤분위삼부주사액조（A조）、정상복소조（B조）、가수술모형조（C조）。협폐기관후8 min（심박취정후약3 min）개시심폐복소，자주순배회복후 A 조급여정맥삼부주사액2.1ml/kg（생리염수희석지5 ml），B 조급여정맥주사주사생리염수5 ml，매격30 min완만정맥주사1차，련속3차；C조정맥부진행삽관등수술조작，불진행호흡심박취정처리화심폐복소조작。관찰복소후삼조동물MAP등변화。자주순배회복후8 h처사동물，취심기조직화뇌해마CA구조직，광경관찰심기화뇌조직적변화、전경관찰초미결구개변。결과재HE염색광경관찰하심기조직화뇌조직적A조화B조지간변화적차이불대，단재전경관찰하칙각조간현시차이교대，A조적손해상대교소。결론심폐복소시응용삼부주사액가이감경심기화뇌조직초미결구손해，대복소후심기화뇌조직유일정적보호작용。
Objective To explore the protective effect of myocardium and hippocampus ultrastructure with Shenfu injection in the cardiopulmonary resuscitation. Methods Sudden cardiac arrest cardiopulmonary resuscitation models were established by adopting the method of clipping on 24 rabbits who were divided into 3 groups, group A which was injected with SF (2.1ml/kg) after successful resuscitated (SF group), group B with NS (5ml), and group C with nothing done. The treatment was repeated 3 times in every 30 minutes. Changes in the MAP during the arrest were observed. The changes in myocardium and hippocampus histiocyte and myocardium and hippocampus ultrastructure were observed with microscope on the rabbits who were executed after 8 hours of successful resuscitation. Results No significant difference was found in myocardium and hippocampus histiocyte between group A and B under the coloring microscope. The myocardium and hippocampus ultrastructure of group B reflected worse than group A, and no change was found in group C. Conclusion SF injection is considered being effective in protecting myocardium and hippocampus from sudden cardiac arrest cardiopulmonary resuscitation.
