大豆科技
大豆科技
대두과기
SOYBEAN BULLETIN
2014年
1期
31-37
,共7页
张彬彬%杨春华%窦博鑫%刘琳琳%邹丽宏%石彦国%刘颖
張彬彬%楊春華%竇博鑫%劉琳琳%鄒麗宏%石彥國%劉穎
장빈빈%양춘화%두박흠%류림림%추려굉%석언국%류영
米曲霉%中性蛋白酶活力%发酵条件
米麯黴%中性蛋白酶活力%髮酵條件
미곡매%중성단백매활력%발효조건
Aspergillus oryzae%Neutral protease activity%Fermentation condition
运用现代生物学技术，对米曲霉40214菌株发酵产中性蛋白酶的培养基组分及培养条件进行优化，旨在提高菌株产中性蛋白酶的产量。通过单因素实验和正交实验确定了发酵培养基的最佳组分，添加葡萄糖8.0%，酵母粉4.0%， Na2HPO40.10%；最适发酵条件为发酵温度28℃，初始pH值6.5，发酵时间96 h，加水量60.0%，经过优化后酶活性提高了1.75倍，达到2845.68 U/mL，实验为进一步提高米曲霉40214菌株产中性蛋白酶的能力并将其应用到食品发酵中奠定了理论基础。
運用現代生物學技術，對米麯黴40214菌株髮酵產中性蛋白酶的培養基組分及培養條件進行優化，旨在提高菌株產中性蛋白酶的產量。通過單因素實驗和正交實驗確定瞭髮酵培養基的最佳組分，添加葡萄糖8.0%，酵母粉4.0%， Na2HPO40.10%；最適髮酵條件為髮酵溫度28℃，初始pH值6.5，髮酵時間96 h，加水量60.0%，經過優化後酶活性提高瞭1.75倍，達到2845.68 U/mL，實驗為進一步提高米麯黴40214菌株產中性蛋白酶的能力併將其應用到食品髮酵中奠定瞭理論基礎。
운용현대생물학기술，대미곡매40214균주발효산중성단백매적배양기조분급배양조건진행우화，지재제고균주산중성단백매적산량。통과단인소실험화정교실험학정료발효배양기적최가조분，첨가포도당8.0%，효모분4.0%， Na2HPO40.10%；최괄발효조건위발효온도28℃，초시pH치6.5，발효시간96 h，가수량60.0%，경과우화후매활성제고료1.75배，체도2845.68 U/mL，실험위진일보제고미곡매40214균주산중성단백매적능력병장기응용도식품발효중전정료이론기출。
Using modern biological technology, medium compositions and fermentation conditions for producing more neutral protease through the cultivation of Aspergillus oryzae were optimized. With single factor trial and orthogonal experiment, the optimal medium compositions determined were: 8.0%glucose, 4.0% yeast extract and 0.10% Na2HPO4. The optimal fermentation conditions obtained were: culture temperature of 28℃, the initial pH of 6.5 and cultivation time of 96 h, 60.0%addition of water. The activity of neutral protease was enhanced to 2 845.68 U/mL. The results provide a theoretical foundation for improving neutral protease production and application in food fermentation of Aspergillus oryzae 40214.
