CAJ | 학술논문

目的：应用不同剂量伴刀豆球蛋白 A（ConA）诱导三种不同品系小鼠急性肝损伤，比较肝酶变化、死亡率和肝组织病理学变化。方法分别用6、12、20和30 mg·kg-1 ConA 处理 C57BL/6J、BABL/c 和 KM 小鼠，8 h 后对比观察急性肝损伤动物血清酶学及肝组织病理学变化情况。结果在6 mg·kg-1 ConA 的作用下，三个品系小鼠均被成功制造出急性肝损伤模型；在12 mg·kg-1 ConA 作用下，KM 小鼠血清 ALT 和AST 分别为（1006.8±12.1） U/L 和（1391.8±13.4）U/L，显著高于 BABL/c 小鼠[（75.7±0.5）U/L 和（284.7±23.4） U/L）和 C57BL/6J 小鼠（104.4±32.2）U/L 和（492.2±12.3）U/L，P 均<0.05]；同样，KM 小鼠肝指数和脾指数分别为（5.4±0.3）和（0.7±0.5），均显著高于 BABL/c 小鼠[（5.2±0.4）和（0.6±0.3）和 C57BL/6J 小鼠（5.0±0.6）和（0.6±0.2），P 均<0.05]；在20 mg·kg-1 ConA 作用下，三组小鼠血清 AST 和 ALT 水平无统计学差异，但 BABL/c 小鼠（4/10）和 C57BL/6J 小鼠（5/10）死亡率显著高于 KM 小鼠（1/10）；在30 mg·kg-1 ConA 作用下，三个品系小鼠死亡率均较高（KM 小鼠为30%，BABL/c 和C57BL/6J 小鼠均为100%）。结论不同品系的小鼠对 ConA 的耐受性不同，KM 小鼠对 ConA 的耐受性明显优于BABL/c 小鼠和 C57BL/6J 小鼠，且呈剂量依赖性。
목적：응용불동제량반도두구단백 A（ConA）유도삼충불동품계소서급성간손상，비교간매변화、사망솔화간조직병이학변화。방법분별용6、12、20화30 mg·kg-1 ConA 처리 C57BL/6J、BABL/c 화 KM 소서，8 h 후대비관찰급성간손상동물혈청매학급간조직병이학변화정황。결과재6 mg·kg-1 ConA 적작용하，삼개품계소서균피성공제조출급성간손상모형；재12 mg·kg-1 ConA 작용하，KM 소서혈청 ALT 화AST 분별위（1006.8±12.1） U/L 화（1391.8±13.4）U/L，현저고우 BABL/c 소서[（75.7±0.5）U/L 화（284.7±23.4） U/L）화 C57BL/6J 소서（104.4±32.2）U/L 화（492.2±12.3）U/L，P 균<0.05]；동양，KM 소서간지수화비지수분별위（5.4±0.3）화（0.7±0.5），균현저고우 BABL/c 소서[（5.2±0.4）화（0.6±0.3）화 C57BL/6J 소서（5.0±0.6）화（0.6±0.2），P 균<0.05]；재20 mg·kg-1 ConA 작용하，삼조소서혈청 AST 화 ALT 수평무통계학차이，단 BABL/c 소서（4/10）화 C57BL/6J 소서（5/10）사망솔현저고우 KM 소서（1/10）；재30 mg·kg-1 ConA 작용하，삼개품계소서사망솔균교고（KM 소서위30%，BABL/c 화C57BL/6J 소서균위100%）。결론불동품계적소서대 ConA 적내수성불동，KM 소서대 ConA 적내수성명현우우BABL/c 소서화 C57BL/6J 소서，차정제량의뢰성。
Objective To compare the differences in serum liver enzymes, mortality and liver histological changes in three different strains of mice with concanavalin A (ConA) -induced acute hepatic injury (AHI). Methods C57BL/6J mice,BABL/c mice and KM mice were subjected to ConA at four different dosages (i.e.6, 12,20 and 30 mg·kg-1) for 8 hours,and then mice were sacrificed and serum liver enzymes and liver histological changes were examined. Results ConA at dose of 6 mg·kg-1 successfully induced acute liver injury in all three strains of mice;ConA at dose of 12 mg·kg-1 caused remarkable increases in serum ALT (1006.8 ± 12.1) U/L and AST (1391.8 ± 13.4)U/L levels in KM mice,significantly higher than those in BABL/c mice [(75.7 ± 0.5) U/L and(284.7 ± 23.4) U/L,respectively and in C57BL/6J mice (104. ±32.2) U/L and (492.2 ± 12.3) U/L,re-spectively,P<0.05];Similarly,liver index(5.4±0.3) and spleen index(0.7±0.5) in KM mice were significantly high-er than that in BABL/c mice [(5.2±0.4) and (0.6±0.3),respectively and in C57BL/6J mice(5.0±0.6) and (0.6±0. 2),respectively,P<0.05];Serum ALT and AST levels did not differ among three strains of mice when Con A at dose of 20 mg·kg -1 was used,however,the mortality in BABL/c (4/10) and C57BL/6J (5/10) was significantly higher than that in KM mice (1/10);30 mg·kg -1 of ConA resulted in a remarkably high mortality in all three strains of mice (30% in KM mice,100% in BABL/c and 100% in C57BL/6J mice). Conclusion Mouse strains play a significant role in liver response to Con A,and the tolerance of KM mice to ConA is better than BABL/c and C57BL/6J mice and the liver damage is in a dose-dependent manner.