CAJ | 학술논문

研究水苏碱对肾纤维化大鼠肾组织CHOP、caspase -3作用的研究．采用单侧输尿管梗阻（ UUO）诱导大鼠肾间质纤维化动物模型，以依那普利为阳性对照药，将大鼠随机分为假手术组、模型组、依那普利组、水苏碱高、中、低剂量组．术后第14 d处死大鼠，收集血清测定血肌酐（ Scr ）、尿素氮（ BUN）；采用HE染色检测肾小管损伤指数，Masson染色对肾间质胶原相对面积进行半定量分析；采用免疫组织化学方法检测肾组织中内质网应激特异转录因子（ CHOP ）、细胞凋亡因子天冬氨酸特异性半胱氨酸蛋白酶3（caspase-3）的表达．各治疗组与模型组比较大鼠肾小管间质损伤指数、肾间质纤维化程度、血清Scr、BUN水平及肾组织CHOP、caspase-3蛋白表达有差异（P＜0．05； P＜0．01）．水苏碱与依那普利组比较，大鼠肾组织CHOP、caspase-3表达降低（P＜0．05），肾小管间质损伤及肾间质纤维化程度明显降低（P＜0．05），肾功能Scr、Bun明显降低（P＜0．05）．水苏碱可能通过下调内质网应激相关凋亡途径CHOP、caspase-3表达，干扰内质网应激特异转录因子的传导通路，抑制细胞凋亡的表达，从而减缓肾间质纤维化的发生和发展．
연구수소감대신섬유화대서신조직CHOP、caspase -3작용적연구．채용단측수뇨관경조（ UUO）유도대서신간질섬유화동물모형，이의나보리위양성대조약，장대서수궤분위가수술조、모형조、의나보리조、수소감고、중、저제량조．술후제14 d처사대서，수집혈청측정혈기항（ Scr ）、뇨소담（ BUN）；채용HE염색검측신소관손상지수，Masson염색대신간질효원상대면적진행반정량분석；채용면역조직화학방법검측신조직중내질망응격특이전록인자（ CHOP ）、세포조망인자천동안산특이성반광안산단백매3（caspase-3）적표체．각치료조여모형조비교대서신소관간질손상지수、신간질섬유화정도、혈청Scr、BUN수평급신조직CHOP、caspase-3단백표체유차이（P＜0．05； P＜0．01）．수소감여의나보리조비교，대서신조직CHOP、caspase-3표체강저（P＜0．05），신소관간질손상급신간질섬유화정도명현강저（P＜0．05），신공능Scr、Bun명현강저（P＜0．05）．수소감가능통과하조내질망응격상관조망도경CHOP、caspase-3표체，간우내질망응격특이전록인자적전도통로，억제세포조망적표체，종이감완신간질섬유화적발생화발전．
This paper investigated the effect of stachydrine on the expression of CHOP ( GAD-Dl53) and caspase-3 in rats with renal interstitial fibrosis .Using unilateral ureter obstruc-tion ( UUO) to induce rats renal intersitital fibrosis animal models , with enalaprilat as the positive control .Rats were randomly divided into control group , model group , enalaprilat group, stachydrine high-dosage groups, stachydrine mid -dosage groups, stachydrine low-dosage groups .Rats were sacrificed after 14 days of operation .Blood were collected to de-termine serum creatinine (Scr) and urea nitrogen (BUN).The degree of the injury in ne-phridial tissue was evaluated by using HE;the degree of renal interstitial fibrosis was calcu-lated by using Masson .The expression of CHOP and caspase -3 in renal tissue was exam-ined by immunohistochemistry .Being compared with the enalaprilat group , expressions of CHOP and caspase -3 in the stachydrine group of high -dosage and mid-dosage were sig-nificantly decreased ( P<0 .05 ); the tubul damage index , renal interstitial fibrosis level , Scr and BUN representing renal function in high -dosage and mid -dosage stachydrine groups were decreased obviously in comparison with those in the enalaprilat group ( P <0.05).Stachydrine might slow down the occurrence and development of RIF by decreasing the expressions of ERS -related CHOP and caspase -3 to interfere in ERS -specific tran-scription factor pathway .The apoptosis was inhibited .