CAJ | 학술논문

目的：探讨不同分期（T2～T4）的尿路膀胱上皮癌（urothelial bladder carcinoma，UBC）中人类表皮生长因子受体-2（HER2）蛋白表达和基因扩增状态。方法：将49例不同分期的膀胱癌患者的肿瘤组织进行HER2免疫组织化学染色，以及HER2基因的双色荧光原位杂交（FISH）检测。结果：膀胱癌患者男性居多。而且分期越高，HER2染色阳性率越高。但所有患者均未出现基因扩增，有12例患者出现17号染色体多倍体。分期越高，多倍体发生越多。结论：膀胱癌患者HER2蛋白增高不是由于基因扩增所致，其它的转录和后转录机制参与并调节了蛋白的表达。
목적：탐토불동분기（T2～T4）적뇨로방광상피암（urothelial bladder carcinoma，UBC）중인류표피생장인자수체-2（HER2）단백표체화기인확증상태。방법：장49례불동분기적방광암환자적종류조직진행HER2면역조직화학염색，이급HER2기인적쌍색형광원위잡교（FISH）검측。결과：방광암환자남성거다。이차분기월고，HER2염색양성솔월고。단소유환자균미출현기인확증，유12례환자출현17호염색체다배체。분기월고，다배체발생월다。결론：방광암환자HER2단백증고불시유우기인확증소치，기타적전록화후전록궤제삼여병조절료단백적표체。
Objective: To explore the expression and gene amplification status of human epidermal growth factor receptor-2 (HER2) in the different stages of invasive urothelial bladder carcinoma. Methods:Tumor tissues from 49 patients with different stages of invasive urothelial bladder carcinoma were tested by immunohistochemical staining for HER2 and HER2 gene fluorescence in situ hybridization. Results:The number of male patients was higher than that of females. The positive rate of HER2 protein expression was higher in the patients with the higher stage of invasive urothelial bladder carcinoma. However, no gene amplification was observed in all patients. Twelve patients had ployploid chromosome 17. More ployploids were observed in the patients with the higher stage of inva-sive urothelial bladder carcinoma. Conclusion:The increase in the protein expression of HER2 in the invasive urothelial bladder carci-noma patients was not caused by gene amplification. Other transcriptional and post-transcriptional mechanisms were probably involved in the regulation of the HER2 protein.