CAJ | 학술논문

为筛选高温淀粉酶高产菌株，利用淀粉酶水解圈作筛选模型，从稻田中采集土样，筛选得到产淀粉酶能力较高的菌株E10。为提高菌株产酶能力，进行了碳源种类及浓度，氮源种类及浓度，无机盐种类及浓度，pH值，种龄及接种量、温度、时间的单因素试验，并对发酵培养基及发酵条件进行了L9(34)正交优化试验。结果表明：最佳产酶发酵培养基为2.0%麸皮、0.1%硝酸钾、0.25%氯化钙，最适初始pH值为6.0。最适产酶条件为种龄24 h，接种量7 mL，于36益下培养48 h。在优化条件下，菌株产酶活力可达180.94 IU/mL。
위사선고온정분매고산균주，이용정분매수해권작사선모형，종도전중채집토양，사선득도산정분매능력교고적균주E10。위제고균주산매능력，진행료탄원충류급농도，담원충류급농도，무궤염충류급농도，pH치，충령급접충량、온도、시간적단인소시험，병대발효배양기급발효조건진행료L9(34)정교우화시험。결과표명：최가산매발효배양기위2.0%부피、0.1%초산갑、0.25%록화개，최괄초시pH치위6.0。최괄산매조건위충령24 h，접충량7 mL，우36익하배양48 h。재우화조건하，균주산매활력가체180.94 IU/mL。
In order to screen a high-yield strain producing thermostable amylase,starch hydrolysis circle was employed as a screening model. The E10 strain achieved from the paddy field soil has high capacity of enzyme production. In order to improve the capability of enzyme production of E10,the single factor experiments was conducted for the kinds and concentrations of carbon source,the kinds and concentrations of nitrogen source,the kinds and concentrations of inorganic salt,pH value,seed age,inoculate volume,temperature and time,then the L9(34)orthogonal optimization test for the fermentation medium and conditions was carried out. The results showed that the best fermentation medium of the enzyme production of E10 was 2.0%wheat bran,0.1%KNO3,0.25%CaCl2,initial pH value of 6.0. The optimal fermentation conditions of the enzyme production of E10 were 24 hours of seed age,7 mL of inoculates volume,36℃of culture temperature,48 hours of time. Under the optimized conditions,the enzyme production of E10 reached 180.94 IU/mL.