CAJ | 학술논문

目的探讨胃癌组织HER2蛋白表达和基因扩增情况及其临床意义.方法收集2011年4月至2012年5月间青岛大学医学院附属医院黄岛院区收治的80例胃癌标本,采用免疫组织化学染色(IHC)和显色原位杂交(CISH)方法检测HER2蛋白表达和基因扩增情况,分析其与临床病理因素的相关性.结果 80例患者中,HER2蛋白表达(-)51例,(+)12例,(++)12例,(+++)5例,阳性表达率为21.3％(17/80)；HER2基因扩增4例,临界扩增3例,基因扩增率为8.8％(7/80).IHC和CISH检测结果呈正相关(P＜0.05),两者的符合率为85.0％(68/80).HER2表达与肿瘤部位、分化程度及TNM分期有关(均P＜0.05).结论胃癌组织中有较高的HER2蛋白表达和基因扩增率,其与胃癌的发生发展密切相关.
목적 탐토위암조직HER2단백표체화기인확증정황급기림상의의.방법 수집2011년4월지2012년5월간청도대학의학원부속의원황도원구수치적80례위암표본,채용면역조직화학염색(IHC)화현색원위잡교(CISH)방법검측HER2단백표체화기인확증정황,분석기여림상병리인소적상관성.결과 80례환자중,HER2단백표체(-)51례,(+)12례,(++)12례,(+++)5례,양성표체솔위21.3％(17/80)；HER2기인확증4례,림계확증3례,기인확증솔위8.8％(7/80).IHC화CISH검측결과정정상관(P＜0.05),량자적부합솔위85.0％(68/80).HER2표체여종류부위、분화정도급TNM분기유관(균P＜0.05).결론 위암조직중유교고적HER2단백표체화기인확증솔,기여위암적발생발전밀절상관.
Objective To explore the clinical significance of protein expression and gene amplification of HER2 in gastric cancer.Methods Immunohistochemistry (IHC) and chromogenic in situ hybridization(CISH) method were used to detect protein expression and gene amplification of HER2 in 80 specimens of gastric cancer patients.Results Protein expression of HER2 was negative in 51 cases,(+) in 12 cases,(++) in 12 cases,(+++) in 5 cases,and the positive expression rate was 21.3％(17/80).Seven (8.8％) cases had gene amplification of HER2,including gene critical amplification in 3(3.8％) cases.The result of IHC was positively correlated with CISH (P＜0.05),and the coincidence rate was 85.0％ (68/80).HER2 positive expression rate was higher in the gastroesophageal junction carcinoma,poorly differentiated and stage Ⅲ-Ⅳ gastric cancer (all P＜0.05).Conclusion The gastric cancer tissue has high positive rate of protein expression and gene amplification of HER2,which is closely correlated to the development of gastric cancer.