中国实用医药
中國實用醫藥
중국실용의약
CHINA PRACTICAL MEDICAL
2014年
7期
9-10,11
,共3页
耿满奎%赵斌%韩龙%岳玺
耿滿奎%趙斌%韓龍%嶽璽
경만규%조빈%한룡%악새
二氧化碳%量压法%酶法
二氧化碳%量壓法%酶法
이양화탄%량압법%매법
Carbon dioxide%Pressure measuring method%Enzymic method
目的：对量压法和酶法检测血清二氧化碳的方法学进行比较,分析两种方法测定血清二氧化碳有无显著性差异。方法依据美国国家临床实验室标准委员会(NCCLS)制定的有关文件,用量压法与酶法分别测定血清二氧化碳100例,运用两种方法检测血清二氧化碳的浓度,计算相关系数r和线性回归方程,并验证两种方法的可比性、精密度和线性关系。结果量压法和酶法相关性比较的回归方程为Y=0.8149X+4.7625,相关系数r=0.9133,差异有统计学意义(P<0.001)；量压法测定血清中的二氧化碳浓度为(22.68±2.03) mmol/L,酶法为(23.24±1.81) mmol/L,说明量压法与酶法测定血清CO2浓度的差异无统计学意义(P>0.001)；两种方法的批内和批间精密度均<5%。结论量压法与酶法测定血清中CO2的相关性、线性和精密度均较好,测定结果具有可比性,可以为临床所接受。
目的：對量壓法和酶法檢測血清二氧化碳的方法學進行比較,分析兩種方法測定血清二氧化碳有無顯著性差異。方法依據美國國傢臨床實驗室標準委員會(NCCLS)製定的有關文件,用量壓法與酶法分彆測定血清二氧化碳100例,運用兩種方法檢測血清二氧化碳的濃度,計算相關繫數r和線性迴歸方程,併驗證兩種方法的可比性、精密度和線性關繫。結果量壓法和酶法相關性比較的迴歸方程為Y=0.8149X+4.7625,相關繫數r=0.9133,差異有統計學意義(P<0.001)；量壓法測定血清中的二氧化碳濃度為(22.68±2.03) mmol/L,酶法為(23.24±1.81) mmol/L,說明量壓法與酶法測定血清CO2濃度的差異無統計學意義(P>0.001)；兩種方法的批內和批間精密度均<5%。結論量壓法與酶法測定血清中CO2的相關性、線性和精密度均較好,測定結果具有可比性,可以為臨床所接受。
목적：대량압법화매법검측혈청이양화탄적방법학진행비교,분석량충방법측정혈청이양화탄유무현저성차이。방법의거미국국가림상실험실표준위원회(NCCLS)제정적유관문건,용량압법여매법분별측정혈청이양화탄100례,운용량충방법검측혈청이양화탄적농도,계산상관계수r화선성회귀방정,병험증량충방법적가비성、정밀도화선성관계。결과량압법화매법상관성비교적회귀방정위Y=0.8149X+4.7625,상관계수r=0.9133,차이유통계학의의(P<0.001)；량압법측정혈청중적이양화탄농도위(22.68±2.03) mmol/L,매법위(23.24±1.81) mmol/L,설명량압법여매법측정혈청CO2농도적차이무통계학의의(P>0.001)；량충방법적비내화비간정밀도균<5%。결론량압법여매법측정혈청중CO2적상관성、선성화정밀도균교호,측정결과구유가비성,가이위림상소접수。
Objective To compare pressure measuring method and enzymic method in detecting carbon dioxide in serum, analyse if there is significant difference in the two methods for determination of carbon dioxide in serum . Methods According to EP9-A file U.S. National Committee for Clinical Laboratory Standards (NCCLS) formulation , the pressure measuring method and enzymic method were used to detect the concentrations of carbon dioxide in serum in 100 cases, calculated the correlation coefficient (r) and linear regression equations ,and verified comparable precision and linearity of the two methods. Results The regression equations related to pressure measuring method and comparison of enzymic method Y=0.8149X+4.7625, Correlation coefficient r=0.9133, the difference was statistically significant(P<0.001);Measurement result of carbon dioxide concentration in serum with pressure measuring method was (22.68±2.03) mmol/L, the one with enzymic method was (23.24±1.81) mmol/L, described pressure measuring method and enzymic method determination of CO2 concentration difference in serum was not statistically significant (P>0.001);Two methods of intra batch and inter batch precision was less than 5%. Conclusion Pressure measuring method and enzymic method are good in determination of serum CO2 correlation, linearity and precision, and measurement results are comparable, which can be accepted by clinically doctors.
