CAJ | 학술논문

为了筛选出漆酶酶活相对较高的菌株，通过愈创木酚变色圈法和定性测定酶活法筛选出高产漆酶菌株 FA-J1，经分子生物学鉴定，菌株属于栓菌属（Trametes）。采用物理诱变法（紫外线诱变）和化学诱变法（硫酸二乙酯）诱变 FA-J1原生质体，最终得到诱变菌株 UV-56和 D52，漆酶酶活提高56％和45％。
위료사선출칠매매활상대교고적균주，통과유창목분변색권법화정성측정매활법사선출고산칠매균주 FA-J1，경분자생물학감정，균주속우전균속（Trametes）。채용물리유변법（자외선유변）화화학유변법（류산이을지）유변 FA-J1원생질체，최종득도유변균주 UV-56화 D52，칠매매활제고56％화45％。
FA-J1 strain with high laccase-producing was screened by guaiacol discoloration circle method and qualitative determination method of enzyme activity and identified as Trametes by ITS.UV-5 6and D52 mutant strains were bred from protoplast of FA-J1 strain treated by UV and diethyl sulfate (DES).The laccase activity of UV-5 6and D52 strains was 56% and 45% higher than FA-J1 strain.