贵州农业科学
貴州農業科學
귀주농업과학
GUIZHOU AGRICULTURAL SCIENCES
2014年
2期
56-58
,共3页
王娅玲%李维峰%杜华波%何素明
王婭玲%李維峰%杜華波%何素明
왕아령%리유봉%두화파%하소명
铅胁迫%咖啡幼苗%叶绿素含量%抗氧化酶活性
鉛脅迫%咖啡幼苗%葉綠素含量%抗氧化酶活性
연협박%가배유묘%협록소함량%항양화매활성
lead stress%coffee seedling%chlorophyll content%antioxidant enzyme activity
为咖啡的抗重金属胁迫机制研究提供理论依据,采用水培方法,在不同浓度梯度铅胁迫下研究咖啡幼苗超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、丙二醛(MDA)以及叶绿素含量的变化。结果表明:铅浓度较低时,咖啡幼苗叶绿素含量略有增加,但随着铅浓度升高,叶绿素含量呈下降趋势;CAT、POD 活性呈先升高后下降趋势,而 SOD 活性随着铅浓度的增加逐渐下降,MDA 含量则一直呈现增加趋势,说明咖啡幼苗膜系统受到了一定程度的破坏。
為咖啡的抗重金屬脅迫機製研究提供理論依據,採用水培方法,在不同濃度梯度鉛脅迫下研究咖啡幼苗超氧化物歧化酶(SOD)、過氧化物酶(POD)、過氧化氫酶(CAT)、丙二醛(MDA)以及葉綠素含量的變化。結果錶明:鉛濃度較低時,咖啡幼苗葉綠素含量略有增加,但隨著鉛濃度升高,葉綠素含量呈下降趨勢;CAT、POD 活性呈先升高後下降趨勢,而 SOD 活性隨著鉛濃度的增加逐漸下降,MDA 含量則一直呈現增加趨勢,說明咖啡幼苗膜繫統受到瞭一定程度的破壞。
위가배적항중금속협박궤제연구제공이론의거,채용수배방법,재불동농도제도연협박하연구가배유묘초양화물기화매(SOD)、과양화물매(POD)、과양화경매(CAT)、병이철(MDA)이급협록소함량적변화。결과표명:연농도교저시,가배유묘협록소함량략유증가,단수착연농도승고,협록소함량정하강추세;CAT、POD 활성정선승고후하강추세,이 SOD 활성수착연농도적증가축점하강,MDA 함량칙일직정현증가추세,설명가배유묘막계통수도료일정정도적파배。
To provide a theoretical reference for investigating the resistance mechanism to heavy metal of coffee seedling,water culture method was used to study the transformation of chlorophyll,SOD,POD, CAT and MDA with different concentrations of lead.The results showed that the contents of chlorophyll slightly increased under low lead concentrations,but decreased when lead concentrations further increased. The activities of CAT and POD went up at the beginning of lead stress,but they were decreased as the quantity of lead increasing.The activities of SOD showed the downtrend as the quantity of lead increasing, while the MDA content increased all the time,which suggested that the membrane system of coffee seedling was damaged by lead stress.