CAJ | 학술논문

为探明白花蛇舌草提取物的体外抗氧化活性，以甲醇、丙酮、80％乙醇为溶剂，采用超声提取、搅拌提取、回流提取法对白花蛇舌草进行提取，考察其对 DPPH（1，1-二苯基-2-三硝基苯肼）自由基活性的清除能力，并与多种抗氧化剂的清除能力进行对比。结果表明：在3种提取方法中，白花蛇舌草80％乙醇回流法提取物的抗氧化活性最强。3种不同溶剂回流提取物与其他5种抗氧化剂清除 DPPH 自由基活性的能力为对香豆酸＜丙酮提取物＜甲醇提取物＜香草酸＜80％乙醇提取物＜芦丁＜咖啡酸＜没食子酸。
위탐명백화사설초제취물적체외항양화활성，이갑순、병동、80％을순위용제，채용초성제취、교반제취、회류제취법대백화사설초진행제취，고찰기대 DPPH（1，1-이분기-2-삼초기분정）자유기활성적청제능력，병여다충항양화제적청제능력진행대비。결과표명：재3충제취방법중，백화사설초80％을순회류법제취물적항양화활성최강。3충불동용제회류제취물여기타5충항양화제청제 DPPH 자유기활성적능력위대향두산＜병동제취물＜갑순제취물＜향초산＜80％을순제취물＜호정＜가배산＜몰식자산。
In order to study the in vitro antioxidant activity of H .diffusa extracts,different solvents including methanol,acetone and 80% alcohol and different methods such as ultrasonic wave extraction, stirring extraction and reflux extraction were investigated.The scavenging capacity on DPPH free radical activity in different H . diffusa extracts,and their comparisons with multiple antioxidants were researched.The results showed that reflux extraction with 80% alcohol was the optimal method,the scavenging capacity on DPPH free radical activity of five antioxidants and H .diffusa extracts with reflux extraction in three different solvents were as follows:P-coumaric acid < acetone extract < methanol extract<vanillic acid <80% alcohol extract<rutin<caffeic acid<gallic acid.