CAJ | 학술논문

为给楠木及其近缘属植物的 DNA 分子鉴定提供依据，对30种楠木及其近缘属植物进行基因组 DNA 提取，叶绿体 matK 序列扩增与测定，并进行序列比对与人工校正，计算属间及种间的遗传距离，比较序列间的差异，构建系统发育树。结果表明：matK 各基因序列的组成中 G＋C 含量平均为36．6％。30个楠木及近缘属植物共发现72个碱基变异位点，碱基变异率为9．38％。其 matK 基因序列种间遗传距离为0～0．098，平均遗传距离为0．010。聚类分析，新樟属为1个分支，楠属和润楠属聚为1个分支，其余樟属、木姜子属、新木姜子属、山胡椒属、檫木属、月桂属与黄肉楠属聚为1个分支。故 matK 序列基本可以对楠木及其近缘属植物进行属间鉴定，但在种间水平上并不可行。
위급남목급기근연속식물적 DNA 분자감정제공의거，대30충남목급기근연속식물진행기인조 DNA 제취，협록체 matK 서렬확증여측정，병진행서렬비대여인공교정，계산속간급충간적유전거리，비교서렬간적차이，구건계통발육수。결과표명：matK 각기인서렬적조성중 G＋C 함량평균위36．6％。30개남목급근연속식물공발현72개감기변이위점，감기변이솔위9．38％。기 matK 기인서렬충간유전거리위0～0．098，평균유전거리위0．010。취류분석，신장속위1개분지，남속화윤남속취위1개분지，기여장속、목강자속、신목강자속、산호초속、찰목속、월계속여황육남속취위1개분지。고 matK 서렬기본가이대남목급기근연속식물진행속간감정，단재충간수평상병불가행。
To provide references for DNA identification of Phoebe and its related genera,genomic DNA was extracted from 30 accessions,the chloroplast matK sequences were analyzed,the inter-specific genetic distances were calculated,and the phylogenetic trees of all the accessions tested were constructed based on the obtained distance data.The results indicated that the average G+C content in various matK sequences was 36.6%.72 base variation points were found,accounting for 9.38%.The interspecific genetic distance ranged from 0 to 0.098,with an average of 0.010.The cluster analysis showed that the genera tested were clustered into three branches,one was Neocinnamomum delavayi ,one was Phoebe and Machilus,and others belonged to another one,including Cinnamomum,Litsea,Neolitsea,Lindera,Sassafras,Laurus and Actinodaphne.matK could be used to identify Phoebe and its related genera,but couldn't be used at the species level.