CAJ | 학술논문

为了开发牧草资源，对贵州部分野生草本植物种质资源的遗传多样性进行研究。根据模式植物拟南芥18S rRNA 基因序列设计特异性引物，对贵州大学农场试验田自然生长的19种野生草本植物的18S rRNA 基因序列进行扩增、测序、构建进化树。结果表明：将获得的1000 bp 左右的 DNA 片段测序进行同源比对，共找到2280个碱基变异位点，分布在8个区段。据各样本18S rRNA 基因的遗传距离构建进化树推测，菊科、苋科和藜科之间存在较近的遗传相似性，豆科中三叶草属与豌豆属之间有较近的遗传距离。
위료개발목초자원，대귀주부분야생초본식물충질자원적유전다양성진행연구。근거모식식물의남개18S rRNA 기인서렬설계특이성인물，대귀주대학농장시험전자연생장적19충야생초본식물적18S rRNA 기인서렬진행확증、측서、구건진화수。결과표명：장획득적1000 bp 좌우적 DNA 편단측서진행동원비대，공조도2280개감기변이위점，분포재8개구단。거각양본18S rRNA 기인적유전거리구건진화수추측，국과、현과화려과지간존재교근적유전상사성，두과중삼협초속여완두속지간유교근적유전거리。
In order to explore forage resource,the genetic diversity of 18 S rRNA gene in 19 kinds of wild herb germplasms were investigated,which were collected from the farm of Guizhou Unversity.The results showed that about 1000 bp fragments of 18 S rRNA genes were amplificated using specific primers based on the gene of Arabidopsis thaliana.After sequencing and homologous comparison,a total of 2 280 nucleotides were found out to be polymorphim sites.Phylogenetic tree of each family were constructed by similarity of 18S rRNA gene.The molecular classification of 19 kinds of wild herbs was consistent with its category based on morphological characteristics.Furthermore,the molecular classification could be useful to distinguish those similar species in morphology, and the genetic data suggested a close genetic relationship in three families,Compositae,Amaranthaceae and Chenopodiaceae.Trifolium and Pisum might share a high genetic similarty with each other.