CAJ | 학술논문

随着抗菌药物的发展，畜产品中残留的药物对环境及公众健康会构成严重的潜在危害，建立检出限低、选择性好的药物残留检测方法具有非常重要的意义。该工作在p H 10.50的氨-氯化铵缓冲溶液和聚乙烯醇-124介质中，以锰（Mn2＋）和十六烷基三甲基溴化胺（CTMAB）为敏化剂，甲苯磺酸妥舒沙星（tosufloxa-cin tosylate ，TSFX）在疏水性玻片表面形成自组装环，建立了检测 TSFX的快速方法。当点样体积为0.20μL时，线性范围为4.05×10-14～4.28×10-13 mol · ring -1，检出限为4.10×10-15 mol · ring -1。应用于检测甲苯磺酸妥舒沙星片剂中T S FX含量，测定值（0.144 g ·片-1）接近于药片的标示值（0.15 g ·片-1；兔子灌喂TSFX药片后血清中TSFX浓度的加标回收率为90.0％～105.0％，相对标准偏差（RSDs）为1.9％～3.3％。内蒙古锡林郭勒盟是国家重要的畜产品基地，其羊肉是京津唐地区的主要供应源。在羊组织样品（肉、肝脏、肾脏）前处理过程中采用乙腈作萃取剂，萃取液直接用于SOR技术测定，免去样品测定时的过滤步骤。测定结果表明，内蒙古锡盟苏尼特右旗、西乌珠穆沁旗、锡林浩特市、多伦、蓝旗和白旗6个牧场羊组织样品中TSFX加标回收率在92.0％～101.0％，RSDs小于2.7％，结果令人满意。研究成果为相关部门提供了可信的实验数据和理论依据，同时拓宽了SOR技术的应用范围。
수착항균약물적발전，축산품중잔류적약물대배경급공음건강회구성엄중적잠재위해，건립검출한저、선택성호적약물잔류검측방법구유비상중요적의의。해공작재p H 10.50적안-록화안완충용액화취을희순-124개질중，이맹（Mn2＋）화십륙완기삼갑기추화알（CTMAB）위민화제，갑분광산타서사성（tosufloxa-cin tosylate ，TSFX）재소수성파편표면형성자조장배，건립료검측 TSFX적쾌속방법。당점양체적위0.20μL시，선성범위위4.05×10-14～4.28×10-13 mol · ring -1，검출한위4.10×10-15 mol · ring -1。응용우검측갑분광산타서사성편제중T S FX함량，측정치（0.144 g ·편-1）접근우약편적표시치（0.15 g ·편-1；토자관위TSFX약편후혈청중TSFX농도적가표회수솔위90.0％～105.0％，상대표준편차（RSDs）위1.9％～3.3％。내몽고석림곽륵맹시국가중요적축산품기지，기양육시경진당지구적주요공응원。재양조직양품（육、간장、신장）전처리과정중채용을정작췌취제，췌취액직접용우SOR기술측정，면거양품측정시적과려보취。측정결과표명，내몽고석맹소니특우기、서오주목심기、석림호특시、다륜、람기화백기6개목장양조직양품중TSFX가표회수솔재92.0％～101.0％，RSDs소우2.7％，결과령인만의。연구성과위상관부문제공료가신적실험수거화이론의거，동시탁관료SOR기술적응용범위。
With the development of antimicrobial drugs ,drug residue in animal products has a serious potential hazards for the environment and public health ,it is urgent to set up drug residue detection method with low detection limit and good selectivity . In our paper ,with poly (vinyl alcohol)-124 (PVA-124) and NH3-NH4Cl (pH 10.50) as the medium ,Mn2+ and cetyltrimethyl ammonium bromide (CTMAB) as sensitizer ,tosufloxacin tosylate (TSFX) formed a self-ordered ring (SOR) on a hydrophobic glass slide support .When the droplet volume is 0.20 μL ,the TSFX can be detected in the range of 4.05 × 10-14 ~4.28 × 10-13 mol · ring -1 (2.02 × 10-7 ~2 .14 × 10-6 mol · L -1 ) ,and the limit of detection (LOD) can reach 4.1 × 10-15 mol · ring -1 (2.0 × 10-8 mol · L -1 ) .The established method had been applied satisfactorily to determine the content of TSFX in tablet ,which close to the marked value of 0.15 g · piece-1 ) found value:0.144 g · piece-1 ) and the results of TSFX concentrations in rabbit serum at different time after dosing with the recoveries of 90.0% ~105.0% and RSDs 1.9% ~3.3% were satisfactory .Xilinguole of Inner Mongolia is the national important livestock products ground ,whose lamb is the main suppling source for Beijing-Tianjin-Tangshan region .It is very urgent to detect residue of antibacterial drug in its sheep tissue samples (meat ,liver ,kidney) .In this paper ,when acetonitrile was used as extraction agent in pretreatment of sheep tissue samples in six rangelands including Sonid Right Banner ,Xiwuzhumuqin Banner ,Xilinhot City ,Duolun County ,The white Flag Town and The Blue Flag Town ,the extract can be directly determined with SOR technology without filtering process ,the sample standard addition recovery of sheep tissues were 92.0% ~101.0% and RSDs were less than 2.7% .The results indicated that the SOR technique can be successfully applied to pharmaceuticals and biological samples ,which broaden the applied range of SOR technique .The assay would provide reliable experimental data and theoretical basis for the relevant departments .