CAJ | 학술논문

血红蛋白发挥多种重要生理功能，但对其结构及功能的认识尚不能满足疾病诊治的需求。拉曼光谱在血红蛋白结构及功能研究中具有很大应用潜力，不但可以检测血红蛋白中血红素及其周围分子结构变化，还可以反映血红蛋白反应动力学具体过程。同时，血红蛋白拉曼光谱在疾病状态下异常血红蛋白检测，血氧饱和度定量测定及血液代用品的高铁血红蛋白含量检测中突显优势。本文综述了拉曼光谱在血红蛋白结构及功能领域中的研究，简述了拉曼光谱在一些病变血红蛋白诊断中的研究进展，分析了影响血红蛋白拉曼光谱检测的因素，以促进拉曼光谱技术在血红蛋白结构和功能研究中的应用。
혈홍단백발휘다충중요생리공능，단대기결구급공능적인식상불능만족질병진치적수구。랍만광보재혈홍단백결구급공능연구중구유흔대응용잠력，불단가이검측혈홍단백중혈홍소급기주위분자결구변화，환가이반영혈홍단백반응동역학구체과정。동시，혈홍단백랍만광보재질병상태하이상혈홍단백검측，혈양포화도정량측정급혈액대용품적고철혈홍단백함량검측중돌현우세。본문종술료랍만광보재혈홍단백결구급공능영역중적연구，간술료랍만광보재일사병변혈홍단백진단중적연구진전，분석료영향혈홍단백랍만광보검측적인소，이촉진랍만광보기술재혈홍단백결구화공능연구중적응용。
Hemoglobin plays many significant biological roles in organism . However ,our knowledge about its structure and function is not enough to meet the demand of clinical diagnosis .Raman spectroscopy has been shown to be an attractive optical technique which can provide direct access to the structure and function of hemoglobin .It is a proven tool for elucidating structur-al information of hemes and other vicinal groups .Furthermore ,it can provide a useful monitor for hemoglobin dynamics . Besides ,Raman spectroscopy has notable advantages in the fields of abnormal hemoglobin diagnosis ,hemoglobin oxygen satura-tion determination and blood methemoglobin analysis .The present paper reviews the research on hemoglobin structure and func-tion using Raman spectroscopy and the application in hemoglobinopathy diagnosis .In addition ,we discuss the factors affecting the measurement in Raman spectroscopy of hemoglobin .The aim of the review is to promote the application of Raman spectros-copy to the research of hemoglobin structure and function .