CAJ | 학술논문

采用紫外-可见光谱（UV-Vis）和傅里叶变换红外光谱（FTIR）法分析辣椒红素在外源活性氧·O2-，H2O2和·OH以及在POD，CAT和LOX防御体系中的结构变化特征。结果表明，外源活性氧处理后辣椒红素最大紫外-可见光吸收峰均出现蓝移现象，其中·O2-和·OH处理后产物FTIR谱峰数减少，峰强变弱，峰形变宽；与色素分子中的羰基和环外烯基发生氧化反应生成烷基和羟基，·OH对色素分子CC具有加成作用；H2O2处理后色素特征峰和强势峰均向低波数位移，产物中含环氧醚基团；在H2O2＋CAT/POD防御体系中辣椒红素UV-Vis和FTIR光谱特征无明显变化；在亚油酸＋LOX体系中色素分子结构发生断裂，产物不含羰基。可见活性氧处理及在LOX底物体系中，辣椒红素分子反应前后UV-Vis和FTIR等光谱特征发生明显变化，色素分子中长烯链断裂，大共轭体系缩短或被破坏，分子中的共轭双键和羰基等发色基团改变，最终生成醇类或醚类等小分子无色物质；而防御酶CAT和POD能减少活性氧对辣椒红素分子的破坏作用。研究结果丰富完善了辣椒色素理论，同时为其开发利用提供了有价值的技术参考依据。
채용자외-가견광보（UV-Vis）화부리협변환홍외광보（FTIR）법분석랄초홍소재외원활성양·O2-，H2O2화·OH이급재POD，CAT화LOX방어체계중적결구변화특정。결과표명，외원활성양처리후랄초홍소최대자외-가견광흡수봉균출현람이현상，기중·O2-화·OH처리후산물FTIR보봉수감소，봉강변약，봉형변관；여색소분자중적탄기화배외희기발생양화반응생성완기화간기，·OH대색소분자CC구유가성작용；H2O2처리후색소특정봉화강세봉균향저파수위이，산물중함배양미기단；재H2O2＋CAT/POD방어체계중랄초홍소UV-Vis화FTIR광보특정무명현변화；재아유산＋LOX체계중색소분자결구발생단렬，산물불함탄기。가견활성양처리급재LOX저물체계중，랄초홍소분자반응전후UV-Vis화FTIR등광보특정발생명현변화，색소분자중장희련단렬，대공액체계축단혹피파배，분자중적공액쌍건화탄기등발색기단개변，최종생성순류혹미류등소분자무색물질；이방어매CAT화POD능감소활성양대랄초홍소분자적파배작용。연구결과봉부완선료랄초색소이론，동시위기개발이용제공료유개치적기술삼고의거。
The changes of UV-Vis and FTIR spectroscopic properties for capsanthin before and after reaction with exogenous su-peroxide anion(· O2-) ,hydrogen peroxide(H2O2 ) and hydroxyl radical(· OH) ,catalase(CAT) ,peroxidase(POD) and lipoxy-genase(LOX) were explored .The results showed that ,the UV-Vis spectral absorption of capsanthin treated with reactive oxy-gen species had a blue-shift .At the same time ,the FTIR spectra changed significantly .The number of FTIR spectral peaks re-duced and theFTIR strength weakened for capsanthin molecule treated with · O2- and · OH .The characteristic and strong peaks moved to shorter wavelengths when treated with H2O2 .And LOX caused breakage of capsanthin molecule and reduction of peak number or groups without carbonyl .Exogenous H2 O2 +CAT or H2 O2 + POD treatment could not affect the UV-Vis and FTIR spectra significantly .So ROS could cause oxidative degradation of capsanthin and destroy chromophoric groups such as carbon-carbon double bond and carbonyl ,then grow colorless alcohols .Hence ROS and LOX should transforms the conjugate system of capsanthin molecules ,while CAT and POD could protect the capsanthin .