CAJ | 학술논문

目的：初步评价15个可变串联重复序列（VNTR）位点对结核分枝杆菌临床分离株的分型能力，寻找适合湖州地区结核分枝杆菌基因分型的位点。方法2011年1-4月连续收集湖州地区结核分枝杆菌临床分离株，采用多位点数目多位点串联系复序列（MLVA）分析方法，对15个VNTR位点进行基因多态性检测，分析单个位点以及15个位点组合的基因分型能力（Hunter-gaston指数，HGDI）。结果分辨率较好的多态性位点有2个：Mtub-21和Mtub -39；分辨率中等的多态性位点共7个：ETR-A，ETR-C，MIRU -10，MIRU -23， MIRU-27，MIRU-40和Mtub-30；分辨率较差的多态性位点有6个：ETR-B，ETR-D，ETR-E，MIRU-16和MIRU-26和MIRU-39。通过计算，本次研究运用15个位点进行基因分型的 HGDI 为0.99，分辨能力好。结论 VNTR位点对结核分枝杆菌基因的分辨力较好，但还需继续寻找其他丰富多态性的VNTR位点，以便快速分型，提高分辨力。
목적：초보평개15개가변천련중복서렬（VNTR）위점대결핵분지간균림상분리주적분형능력，심조괄합호주지구결핵분지간균기인분형적위점。방법2011년1-4월련속수집호주지구결핵분지간균림상분리주，채용다위점수목다위점천련계복서렬（MLVA）분석방법，대15개VNTR위점진행기인다태성검측，분석단개위점이급15개위점조합적기인분형능력（Hunter-gaston지수，HGDI）。결과분변솔교호적다태성위점유2개：Mtub-21화Mtub -39；분변솔중등적다태성위점공7개：ETR-A，ETR-C，MIRU -10，MIRU -23， MIRU-27，MIRU-40화Mtub-30；분변솔교차적다태성위점유6개：ETR-B，ETR-D，ETR-E，MIRU-16화MIRU-26화MIRU-39。통과계산，본차연구운용15개위점진행기인분형적 HGDI 위0.99，분변능력호。결론 VNTR위점대결핵분지간균기인적분변력교호，단환수계속심조기타봉부다태성적VNTR위점，이편쾌속분형，제고분변력。
Objective To preliminarily evaluate the ability of 15 -loci variable number tandem repeats on the genotyping of clinical isolates of mycobacterium tuberculosis,and explore the suitable loci for genotyping of Mycobacterium tuberculosis in Huzhou City.Methods From 201 1 January to 201 1 April,the clinical isolates of mycobacterium tuberculosis were selected from Huzhou area to test the polymorphism of every loci by using MLVA detection method and analyze hunter-gaston discriminatory index of individual locus as well as combinations of 15 locus.Results There were two locus of Mtub-21 and Mtub-39 which had better degree of polymorphism;seven locus of ETR-A,ETR-C,MIRU-10,MIRU-23,MIRU-27,MIRU-40 and Mtub-30 had moderate degree of polymorphism;six locus of ETR-B,ETR-D,ETR-E,MIRU-16,MIRU-26 and MIRU-39 had poor degree of polymorphism.By calculation,the result showed that HGDI was 0.99 by using 15 locus to genotype,which indicated the ability to distinguish the genotype was good.Conclusion We will continue to look for other rich VNTR polymorphism locus to fit the local region fast genotyping of mycobacterium tuberculosis for higher resolution and cost effectiveness of mycobacterium tuberculosis control and prevention.