CAJ | 학술논문

目的探讨硒-甲基硒代半胱氨酸( MSC)对乳腺癌MCF-7细胞抑制及其诱导凋亡的作用机制。方法采用不同浓度(12.5、25、50、100、200μmol/L ) MSC 作用于 MCF-7细胞,四甲基偶氮唑盐( MTT)法测定MSC对细胞增殖的抑制作用；Hoechst染色法观察细胞凋亡形态；氮蓝四唑( NBT)显色法检测MSC对细胞超氧化物歧化酶( SOD)活性的影响；硫代巴比妥酸法( TBA )法检测 MSC 对细胞内丙二醛( MDA)水平的影响；RT-PCR法检测细胞Survivin基因的表达。结果MSC对MCF-7细胞的增殖具有抑制作用,细胞存活率随其浓度的增加而逐渐降低；荧光染色可见,MSC干预组细胞变圆、胞核皱缩、染色质浓缩,与对照组比较差异有统计学意义；MSC降低MCF-7细胞内SOD的活性,升高细胞内MDA水平( P<0.01)；同时, MSC能下调Survivin mRNA的表达( P<0.01)。结论MSC可通过调节乳腺癌细胞内氧化还原状态及Survivin基因的表达抑制乳腺癌细胞的体外增值和诱导凋亡, MSC作为一种新型营养强化剂有望开发其预防和辅助乳腺癌治疗的新用途。
목적탐토서-갑기서대반광안산( MSC)대유선암MCF-7세포억제급기유도조망적작용궤제。방법채용불동농도(12.5、25、50、100、200μmol/L ) MSC 작용우 MCF-7세포,사갑기우담서염( MTT)법측정MSC대세포증식적억제작용；Hoechst염색법관찰세포조망형태；담람사서( NBT)현색법검측MSC대세포초양화물기화매( SOD)활성적영향；류대파비타산법( TBA )법검측 MSC 대세포내병이철( MDA)수평적영향；RT-PCR법검측세포Survivin기인적표체。결과MSC대MCF-7세포적증식구유억제작용,세포존활솔수기농도적증가이축점강저；형광염색가견,MSC간예조세포변원、포핵추축、염색질농축,여대조조비교차이유통계학의의；MSC강저MCF-7세포내SOD적활성,승고세포내MDA수평( P<0.01)；동시, MSC능하조Survivin mRNA적표체( P<0.01)。결론MSC가통과조절유선암세포내양화환원상태급Survivin기인적표체억제유선암세포적체외증치화유도조망, MSC작위일충신형영양강화제유망개발기예방화보조유선암치료적신용도。
Objective To investigate the effect of Se-methylselenoeystein( MSC) on the proliferation and apoptosis in breast cancer cell line MCF-7 . Methods The human breast cancer MCF-7 cells were treated with different con-centrations MSC:12.5, 25, 50, 100, 200μmol/L. The cell viability was examined by methyl thiazolyl tetrazolium ( MTT) assay, the apoptosis detected by Hoechst stain, the activity of superoxide dismutase( SOD) determined by nitrotetrazolium blue chloride ( NBT ) , and the level of malondialdehyde ( MDA ) detected by thiobarbituric acid ( TBA) . The expression of survivin gene in MCF-7 cells was test by RT-PCR. Results The proliferation of MCF-7 cells was inhibited by MSC, the cell viability reduced gradually with MSC concentration increased(P<0.01). In the MSC pretreatment group, it showed cell rounded, nuclear shrinkage, chromatin concentration by fluorescence staning. MSC could decrease the activity of SOD, and increase the level of MDA(P<0.01). The survivin gene ex-pression in MSC pretreatment group was lower than that of control group(P<0.01). Conclusion MSC can inhibit the proliferation of MCF-7 cells through regulating oxidative statues and the expression of survivin gene. MSC, a kind of new nutrition fortification, is being expected to be a new material to prevent and treat the breast cancer.