安徽医科大学学报
安徽醫科大學學報
안휘의과대학학보
ACTA UNIVERSITY MEDICINALIS ANHUI
2014年
2期
168-171,172
,共5页
慢性间歇性缺氧%Toll样受体4%c-Jun氨基末端激酶%肿瘤坏死因子-α%瘦素
慢性間歇性缺氧%Toll樣受體4%c-Jun氨基末耑激酶%腫瘤壞死因子-α%瘦素
만성간헐성결양%Toll양수체4%c-Jun안기말단격매%종류배사인자-α%수소
chronic intermittent hypoxia%Toll-like receptor 4%C-Jun N-terminal kinase%tumor necrosis factor-α%Leptin
目的探讨Toll样受体4( TLR4)、c-Jun氨基末端激酶( JNK)、肿瘤坏死因子α( TNF-α)、瘦素( Leptin)与慢性间歇性缺氧( CIH)的关系。方法将24只SD大鼠随机分成3组：对照组、CIH 6周组、CIH 8周组,每组8只。造模成功后,采用无创血压分析仪观察大鼠血压变化,HE染色观察肾动脉病理学改变,采用ELISA法检测血清TNF-α、Leptin的含量,利用实时荧光定量 PCR 技术检测大鼠脂肪组织中TLR4、JNK mRNA表达水平。结果模型组大鼠血压明显高于对照组(P<0.01),且模型组大鼠肾动脉病理变化较对照组显著加重；CIH 6周组和CIH 8周组的血清TNF-α、Lep-tin的含量与对照组比较显著增加( P <0.01),且 TNF-α、Leptin的含量随缺氧天数的增加而显著增加( P <0.01)；CIH 6周组和CIH 8周组的TLR4、JNK mRNA水平比对照组显著增加( P<0.01),且TLR4、JNK mRNA水平随缺氧天数的增加而显著增加( P<0.01)。结论TLR4、JNK、TNF-α、Leptin可能参与了CIH大鼠的炎症反应。
目的探討Toll樣受體4( TLR4)、c-Jun氨基末耑激酶( JNK)、腫瘤壞死因子α( TNF-α)、瘦素( Leptin)與慢性間歇性缺氧( CIH)的關繫。方法將24隻SD大鼠隨機分成3組：對照組、CIH 6週組、CIH 8週組,每組8隻。造模成功後,採用無創血壓分析儀觀察大鼠血壓變化,HE染色觀察腎動脈病理學改變,採用ELISA法檢測血清TNF-α、Leptin的含量,利用實時熒光定量 PCR 技術檢測大鼠脂肪組織中TLR4、JNK mRNA錶達水平。結果模型組大鼠血壓明顯高于對照組(P<0.01),且模型組大鼠腎動脈病理變化較對照組顯著加重；CIH 6週組和CIH 8週組的血清TNF-α、Lep-tin的含量與對照組比較顯著增加( P <0.01),且 TNF-α、Leptin的含量隨缺氧天數的增加而顯著增加( P <0.01)；CIH 6週組和CIH 8週組的TLR4、JNK mRNA水平比對照組顯著增加( P<0.01),且TLR4、JNK mRNA水平隨缺氧天數的增加而顯著增加( P<0.01)。結論TLR4、JNK、TNF-α、Leptin可能參與瞭CIH大鼠的炎癥反應。
목적탐토Toll양수체4( TLR4)、c-Jun안기말단격매( JNK)、종류배사인자α( TNF-α)、수소( Leptin)여만성간헐성결양( CIH)적관계。방법장24지SD대서수궤분성3조：대조조、CIH 6주조、CIH 8주조,매조8지。조모성공후,채용무창혈압분석의관찰대서혈압변화,HE염색관찰신동맥병이학개변,채용ELISA법검측혈청TNF-α、Leptin적함량,이용실시형광정량 PCR 기술검측대서지방조직중TLR4、JNK mRNA표체수평。결과모형조대서혈압명현고우대조조(P<0.01),차모형조대서신동맥병리변화교대조조현저가중；CIH 6주조화CIH 8주조적혈청TNF-α、Lep-tin적함량여대조조비교현저증가( P <0.01),차 TNF-α、Leptin적함량수결양천수적증가이현저증가( P <0.01)；CIH 6주조화CIH 8주조적TLR4、JNK mRNA수평비대조조현저증가( P<0.01),차TLR4、JNK mRNA수평수결양천수적증가이현저증가( P<0.01)。결론TLR4、JNK、TNF-α、Leptin가능삼여료CIH대서적염증반응。
Objective To investigate the association between TLR4,C-Jun N-terminal kinase,tumor necrosis factor-α(TNF-α),Leptin changes and chronic intermittent hypoxia(CIH). Methods Twenty-four SD rats were divided into 3 groups (n=8):control group,CIH 6 weeks group, CIH 8 weeks group. After the modeling,the change of blood pressure and renal artery were observed by the use of non-invasive blood pressure analyzer and HE staining. ELISA was employed to determine the production of TNF-αand Leptin in sera of these animals. The expressions of TLR4 and JNK mRNA were detected by real time quantitativer everse transcription-polymerase chain reaction ( QRT-PCR) method in the adipose tissue. Results The blood pressure in intermittent hypoxia group was signifi-cantly higher than those in control group(P<0.01), and the renal artery pathological changes in intermittent hy-poxia group were significantly heavier than those in control group;the content of TNF-α and Leptin in intermittent hypoxia was significantly higher than those in control group(P<0.01),and the content of TNF-α and Leptin was significantly increased with the days of hypoxia;the expression of TLR4 and JNK at mRNA levels in intermittent hy-poxia was significantly higher than those in control group(P<0.01),and the expression of TLR4 and JNK at mR-NA levels was significantly increased with the days of hypoxia. Conclusion TLR4,JNK,TNF-α,Leptin may be in-volved in the inflammatory response of chronic intermittent hypoxia in rats.
