CAJ | 학술논문

目的基于金纳米笼的光热转换特性,观察经抗体修饰的免疫金纳米笼对人乳腺癌细胞 MCF-7的靶向光热效应,探讨其对肿瘤细胞的杀伤效果及其细胞毒性。方法由银( Ag)立方纳米晶与次氯金酸( HAuCl4)置换反应得到金纳米笼,经Anti-CK19抗体修饰后制成具有生物靶向性的免疫金纳米笼。实验组(A组)：将金纳米笼-抗体探针链接细胞后,由功率密度为1.5 W/cm2、波长为808 nm的激光照射30 min；对照组( B组)分为2组：B玉组经相同光照强度和波长的激光单纯光照30 min；BⅡ组为单纯加金纳米笼无光照。观察A、B组温度随时间变化情况,通过荧光终止剂细胞染色及流式细胞术检测免疫金纳米笼的光热杀伤效应,应用四甲基偶氮唑蓝( MTT)比色法观察其细胞毒性。结果A组温度升高明显,达48℃,而B组温度无明显变化,维持在35℃左右。荧光终止剂细胞染色及流式细胞术显示A组细胞大量凋亡,而B组细胞活性基本未受影响( P<0.05)。 MMT法显示细胞毒性较小( P<0.05)。结论免疫金纳米笼对MCF-7细胞具有靶向杀伤作用,被用于光热治疗。
목적기우금납미롱적광열전환특성,관찰경항체수식적면역금납미롱대인유선암세포 MCF-7적파향광열효응,탐토기대종류세포적살상효과급기세포독성。방법유은( Ag)립방납미정여차록금산( HAuCl4)치환반응득도금납미롱,경Anti-CK19항체수식후제성구유생물파향성적면역금납미롱。실험조(A조)：장금납미롱-항체탐침련접세포후,유공솔밀도위1.5 W/cm2、파장위808 nm적격광조사30 min；대조조( B조)분위2조：B옥조경상동광조강도화파장적격광단순광조30 min；BⅡ조위단순가금납미롱무광조。관찰A、B조온도수시간변화정황,통과형광종지제세포염색급류식세포술검측면역금납미롱적광열살상효응,응용사갑기우담서람( MTT)비색법관찰기세포독성。결과A조온도승고명현,체48℃,이B조온도무명현변화,유지재35℃좌우。형광종지제세포염색급류식세포술현시A조세포대량조망,이B조세포활성기본미수영향( P<0.05)。 MMT법현시세포독성교소( P<0.05)。결론면역금납미롱대MCF-7세포구유파향살상작용,피용우광열치료。
Objective Based on the solar-thermal conversion characteristics of the gold nanocages, we investigated the photothermal effect and ceel toxicity of immune gold nanocage in a human breast cancer cell MCF-7 . Methods Gold nanocages were obtained through the replacement reaction between silver ( Ag ) cubic nanocrystalline and chloroauric acid( HAuCl4 ) . Then the nanoparticles were modified with anti-CK19 antibody to have gold biological targeting characteristics. In experimental group, gold nanocage-antibody probe was applied to the cell, and then treated with the power density 1. 5 W/cm2 , the wavelength of 808 nm laser for 30 min; in control group, gold nanocage-antibody probe was applied to the cell, but no light treatment, or cells were treated with light only. At the end of each treatment, MCF-7 cells were collected for fluorescence termination agent cell staining and flow cytome-try assay for the photothermal effect. The cytotoxicity was examined by colorimetric method of methyl thiazolyl tet-razolium( MTT) . Results The temperature increased significantly to 48 ℃ in control group, but not in control group ( remained 35 ℃) . Cells in experimental group showed significant apoptosis in fluorescent termination agent cell staining and flow cytometry assay, while the cells in control group showed no apoptosis(P<0.05). MMT ex-periments showed that cytotoxicity of this method was small(P<0. 05). Conclusion Immuno gold nanocages have a specific targeting killing effect on MCF-7 cell, and be used for photothermal therapy.