CAJ | 학술논문

背景：鹿茸是惟一能够周期性再生的复杂哺乳动物器官，其再生过程是基于干细胞的存在。研究鹿茸再生机制，探索干细胞在哺乳动物器官再生中的作用对于再生生物学和再生医学研究具有重要的意义。 　　目的：综述鹿茸再生研究，干细胞及相关因子在鹿茸再生中的作用。 　　方法：应用计算机检索1994年1月至2012年10月PubMed 数据库(http：//www.ncbi.nlm.nih.gov/PubMed)。检索词为：deer antler；antler regeneration；stem cel ，并限定文章语言种类为English。此外还手动查阅相关专著数部。纳入文章所述内容涉及鹿茸再生的组织学、形态学、鹿茸干细胞与微环境研究、相关细胞因子。排除重复研究和纳入标准无关的文章。 　　结果与结论：共检索文献87篇，最终纳入文献36篇。决定鹿茸发生及再生的关键组织分别为生茸区骨膜和角柄骨膜，这两种组织中的细胞被定义为鹿茸干细胞。鹿茸干细胞上覆盖的皮肤组织构成了这些干细胞活动所需的特定微环境。多种细胞因子如胰岛素样生长因子、性激素、人表皮生长因子、血管内皮生长因子等参与了鹿茸再生及快速生长调控。探索鹿茸干细胞微环境内各组分间相互作用所需的信号因子、阐明其调控机制，对于揭示解鹿茸再生之谜，对了解干细胞在哺乳动物器官再生的作用具有十分重要的意义。
배경：록용시유일능구주기성재생적복잡포유동물기관，기재생과정시기우간세포적존재。연구록용재생궤제，탐색간세포재포유동물기관재생중적작용대우재생생물학화재생의학연구구유중요적의의。 　　목적：종술록용재생연구，간세포급상관인자재록용재생중적작용。 　　방법：응용계산궤검색1994년1월지2012년10월PubMed 수거고(http：//www.ncbi.nlm.nih.gov/PubMed)。검색사위：deer antler；antler regeneration；stem cel ，병한정문장어언충류위English。차외환수동사열상관전저수부。납입문장소술내용섭급록용재생적조직학、형태학、록용간세포여미배경연구、상관세포인자。배제중복연구화납입표준무관적문장。 　　결과여결론：공검색문헌87편，최종납입문헌36편。결정록용발생급재생적관건조직분별위생용구골막화각병골막，저량충조직중적세포피정의위록용간세포。록용간세포상복개적피부조직구성료저사간세포활동소수적특정미배경。다충세포인자여이도소양생장인자、성격소、인표피생장인자、혈관내피생장인자등삼여료록용재생급쾌속생장조공。탐색록용간세포미배경내각조분간상호작용소수적신호인자、천명기조공궤제，대우게시해록용재생지미，대료해간세포재포유동물기관재생적작용구유십분중요적의의。
BACKGROUND:Deer antlers are the unique mammalian organs which can periodical y regenerate, and the process is known as a stem cel-based event. Exploring the underlying mechanism of deer antler regeneration and indentifying the functional role of stem cellin mammalian organ regeneration are of great importance to regenerative biology and regenerative medicine. OBJECTIVE:To review the relevant literatures of the research progress in antler regeneration, as wel as effects of stem cells and cytokines on antler regeneration. METHODS:A computer-based online search of PubMed (1994-01/2012-10) was performed for acquiring the articles in English by using the key words of“deer antler;antler regeneration;stem cell. In addition, manual search was also performed for those literatures that cannot be readily obtained from internet search. Articles concerning antler regeneration histology, morphology, antler stem cells and micro-environmental studies, and related cytokines. Repetitive studies or articles that are unrelated to the criteria set for the article were excluded. RESULTS AND CONCLUSION:A total of 87 articles were obtained and final y 31 articles were selected. The key tissue types for antler regeneration are antlerogenic periosteum and pedicle periosteum, the cells within which are known as antler stem cells. The covering skin of antlerogenic periosteum and pedicle periosteum constitutes the functional niche for antler stem cells. Numerous cytokines are involved in the process of antler fast growing and ful regeneration, including insulin-like growth factor, sex hormones, human epidermal growth factor, and vascular endothelial growth factor. It is vital y important to identify the interacting molecules between the antler stem cells and their niche celltypes, and to define the role of each molecule that plays in antler regeneration, which wil greatly advance our knowledge of the stem cel-based mammalian organ regeneration.