中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2013年
45期
7891-7897
,共7页
沈丽萍%王帅帅%董丽果%沈霞%花放%叶新春%崔桂云
瀋麗萍%王帥帥%董麗果%瀋霞%花放%葉新春%崔桂雲
침려평%왕수수%동려과%침하%화방%협신춘%최계운
干细胞%脐带脐血干细胞%人脐血间充质干细胞%条件培养基%经鼻给药%脑梗死%神经保护%国家自然科学基金
榦細胞%臍帶臍血榦細胞%人臍血間充質榦細胞%條件培養基%經鼻給藥%腦梗死%神經保護%國傢自然科學基金
간세포%제대제혈간세포%인제혈간충질간세포%조건배양기%경비급약%뇌경사%신경보호%국가자연과학기금
背景:人脐血间充质干细胞分泌细胞因子和神经营养因子对大鼠脑缺血再灌注损伤具有神经保护作用,但经鼻给予人脐血间充质干细胞条件培养基治疗脑卒中的研究报道较少。<br> 目的:观察经鼻给人脐血间充质干细胞条件培养基对脑缺血再灌注损伤大鼠神经功能恢复的影响。<br> 方法:制备成年大鼠右侧大脑中动脉脑缺血再灌注模型,体外分离培养健康人脐血间充质干细胞,制备含有人脐血间充质干细胞分泌物质的条件培养基。造模后大鼠随机分为3组:对照组,单纯培养基对照组和条件培养基治疗组,各组每天经鼻给生理盐水、培养基DMEM/F12、条件培养基(10 mL/kg)治疗,术后1,7,14 d分别进行神经功能缺损评分、足错步试验。<br> 结果与结论:术后第1天,各组神经功能缺损评分、足错步次数比较差异无显著性意义(P>0.05)。术后第7,14天,对照组与单纯培养基组神经功能缺损评分、足错步次数比较,差异无显著性意义(P>0.05)。条件培养基治疗组神经功能缺损评分、足错步次数均明显少于对照组和单纯培养基组,差异有显著性意义(P<0.05)。说明经鼻给人脐血间充质干细胞条件培养基可明显促进缺血再灌注后大鼠神经功能的恢复。
揹景:人臍血間充質榦細胞分泌細胞因子和神經營養因子對大鼠腦缺血再灌註損傷具有神經保護作用,但經鼻給予人臍血間充質榦細胞條件培養基治療腦卒中的研究報道較少。<br> 目的:觀察經鼻給人臍血間充質榦細胞條件培養基對腦缺血再灌註損傷大鼠神經功能恢複的影響。<br> 方法:製備成年大鼠右側大腦中動脈腦缺血再灌註模型,體外分離培養健康人臍血間充質榦細胞,製備含有人臍血間充質榦細胞分泌物質的條件培養基。造模後大鼠隨機分為3組:對照組,單純培養基對照組和條件培養基治療組,各組每天經鼻給生理鹽水、培養基DMEM/F12、條件培養基(10 mL/kg)治療,術後1,7,14 d分彆進行神經功能缺損評分、足錯步試驗。<br> 結果與結論:術後第1天,各組神經功能缺損評分、足錯步次數比較差異無顯著性意義(P>0.05)。術後第7,14天,對照組與單純培養基組神經功能缺損評分、足錯步次數比較,差異無顯著性意義(P>0.05)。條件培養基治療組神經功能缺損評分、足錯步次數均明顯少于對照組和單純培養基組,差異有顯著性意義(P<0.05)。說明經鼻給人臍血間充質榦細胞條件培養基可明顯促進缺血再灌註後大鼠神經功能的恢複。
배경:인제혈간충질간세포분비세포인자화신경영양인자대대서뇌결혈재관주손상구유신경보호작용,단경비급여인제혈간충질간세포조건배양기치료뇌졸중적연구보도교소。<br> 목적:관찰경비급인제혈간충질간세포조건배양기대뇌결혈재관주손상대서신경공능회복적영향。<br> 방법:제비성년대서우측대뇌중동맥뇌결혈재관주모형,체외분리배양건강인제혈간충질간세포,제비함유인제혈간충질간세포분비물질적조건배양기。조모후대서수궤분위3조:대조조,단순배양기대조조화조건배양기치료조,각조매천경비급생리염수、배양기DMEM/F12、조건배양기(10 mL/kg)치료,술후1,7,14 d분별진행신경공능결손평분、족착보시험。<br> 결과여결론:술후제1천,각조신경공능결손평분、족착보차수비교차이무현저성의의(P>0.05)。술후제7,14천,대조조여단순배양기조신경공능결손평분、족착보차수비교,차이무현저성의의(P>0.05)。조건배양기치료조신경공능결손평분、족착보차수균명현소우대조조화단순배양기조,차이유현저성의의(P<0.05)。설명경비급인제혈간충질간세포조건배양기가명현촉진결혈재관주후대서신경공능적회복。
BACKGROUND:Cytokines and neurotrophic factors secreted from human umbilical cord blood-derived mesenchymal stem cells secrete have neuroprotective effects on cerebral ischemia-reperfusion injury, but there are few reports about intranasal administration of human umbilical cord blood-derived mesenchymal stem cellconditioned medium in the treatment of stroke. <br> OBJECTIVE:To investigate the protective effects of intranasal administration of human umbilical cord-derived mesenchymal stem cells-conditioned medium on neurologic function of rats with cerebral ischemia-reperfusion injury. <br> METHODS:Adult rats were subjected to 2 hours of right middle cerebral artery occlusion and the human umbilical cord-derived mesenchymal stem cells were isolated from the postpartum human cord. We made the conditioned medium of human umbilical cord-derived mesenchymal stem cells. Ischemic rats were randomized and assigned to three groups and were treated by intranasal routine starting 24 hours after middle cerebral artery occlusion with:(1) saline for control group;(2) Dulbecco’s modified Eagle’s medium/Ham’s nutrient mixture F-12 medium for medium control group;(3) conditioned medium treatment group (10mL/kg) daily for 14 days. Behavioral tests (foot fault test, and modified Neurological Severity Score) were performed before and at 1, 7, 14 days after middle cerebral artery occlusion. <br> RESULTS AND CONCLUSION:There was no difference in the behavioral tests among the three groups at postoperatively 1 day (P>0.05). Compared to the control and medium control group rats, respectively, rats in the conditioned medium group significantly improved functional outcome after stroke in days 7 and 14 (P<0.05). There was also no significant difference in functional tests between the control group and medium control group in days 7 and 14 (P>0.05). These results suggest that human umbilical cord-derived mesenchymal stem cells-conditioned medium via intranasal administration can significantly improve neurologic functional outcome after cerebral ischemia-reperfusion injury.