CAJ | 학술논문

背景：不同氧分压对人脂肪来源干细胞分泌细胞因子的影响目前尚未定论，这些差异可能由于研究者对于氧分压的选取不同而造成影响。 　　目的：检测不同氧分压对人脂肪来源干细胞分泌细胞因子的影响。 　　方法：体外分离培养人脂肪来源干细胞进行免疫表型进行鉴定。将人脂肪来源干细胞分别在1%，3%，5%，10%，21%氧分压的环境中培养24 h后，使用实时定量PCR和酶联免疫吸附法对人脂肪来源干细胞分泌的血管内皮生长因子、肝细胞生长因子、神经细胞生长因子、角质细胞生长因子，在基因水平以及蛋白水平上进行检测分析。 　　结果与结论：人脂肪来源的人脂肪来源干细胞阳性表达 CD71，CD73，CD90，CD105，阴性表达 CD34， CD45，CD54及HLA-DR。经单因素方差分析统计，在基因水平上，低氧环境(体积分数1%，3%氧)均可促进人脂肪来源干细胞显著性高表达血管内皮生长因子、神经生长因子(P均<0.01)；对人脂肪来源干细胞表达肝细胞生长因子有显著影响(P <0.05)；而对角质细胞生长因子的表达则无显著影响(P >0.05)。在蛋白水平上，低氧促进人脂肪来源干细胞分泌肝细胞生长因子、血管内皮生长因子蛋白(P均<0.01)，对神经生长因子、角质细胞生长因子的分泌无显著影响。结果提示，人脂肪来源干细胞在低氧环境中培养后，其生物学特性受到一定的改变，可促进血管内皮生长因子、肝细胞生长因子、神经生长因子基因的表达，提高肝细胞生长因子、血管内皮生长因子蛋白的分泌。
배경：불동양분압대인지방래원간세포분비세포인자적영향목전상미정론，저사차이가능유우연구자대우양분압적선취불동이조성영향。 　　목적：검측불동양분압대인지방래원간세포분비세포인자적영향。 　　방법：체외분리배양인지방래원간세포진행면역표형진행감정。장인지방래원간세포분별재1%，3%，5%，10%，21%양분압적배경중배양24 h후，사용실시정량PCR화매련면역흡부법대인지방래원간세포분비적혈관내피생장인자、간세포생장인자、신경세포생장인자、각질세포생장인자，재기인수평이급단백수평상진행검측분석。 　　결과여결론：인지방래원적인지방래원간세포양성표체 CD71，CD73，CD90，CD105，음성표체 CD34， CD45，CD54급HLA-DR。경단인소방차분석통계，재기인수평상，저양배경(체적분수1%，3%양)균가촉진인지방래원간세포현저성고표체혈관내피생장인자、신경생장인자(P균<0.01)；대인지방래원간세포표체간세포생장인자유현저영향(P <0.05)；이대각질세포생장인자적표체칙무현저영향(P >0.05)。재단백수평상，저양촉진인지방래원간세포분비간세포생장인자、혈관내피생장인자단백(P균<0.01)，대신경생장인자、각질세포생장인자적분비무현저영향。결과제시，인지방래원간세포재저양배경중배양후，기생물학특성수도일정적개변，가촉진혈관내피생장인자、간세포생장인자、신경생장인자기인적표체，제고간세포생장인자、혈관내피생장인자단백적분비。
BACKGROUND:Effects of different oxygen partial pressures on cytokine secretion of human adipose-derived stem cells have been differently reported. These differences may be caused by varying oxygen partial pressures. OBJECTIVE:To investigate the influence of different oxygen partial pressures on cytokines secreted from human adipose-derived stem cells. METHODS:Human adipose-derived stem cells were cultured in vitro and identified by its immunophenotype. Human adipose-derived stem cells were divided into five groups and cultured under different oxygen partial pressure conditions (1%, 3%, 5%, 10%, 21%) for 24 hours, respectively. With quantitative real-time PCR and enzyme linked immunosorbent assay, the secretion of cytokines, vascular endothelial growth factor, hepatocyte growth factor, nerve growth factor, keratinocyte growth factor, from human adipose-derived stem cells were analyzed on the gene and protein levels. RESULTS AND CONCLUSION:Human adipose-derived stem cells were positive for CD71, CD73, CD90, CD105 and negative for CD34, CD45, CD54, HLA-DR. From the aspect of gene level, hypoxia (1%, 3%O 2 ) promoted the expression of vascular endothelial growth factor and nerve growth factor from human adipose-derived stem cells (P<0.01), and significantly elevated the expression of hepatocyte growth factor (P<0.05);however, there was no significant influence on keratinocyte growth factor under hypoxia (P>0.05). Based on the protein level, protein secretion of hepatocyte growth factor and vascular endothelial growth factor from human adipose-derived stem cells was increased under hypoxia (P<0.01), but no changes occurred in nerve growth factor and keratinocyte growth factor. After cultured under hypoxic environment, human adipose-derived stem cells were promoted to express gene vascular endothelial growth factor, hepatocyte growth factor and nerve growth factor, as wel as to secrete protein keratinocyte growth factor and vascular endothelial growth factor.