CAJ | 학술논문

背景：前期实验成功制备了一种新型半乳糖化海藻酸钠-壳低聚糖微胶囊，国内外尚未见以壳低聚糖和海藻酸钠制备的微胶囊用于肝细胞包裹的研究。 　　目的：研究半乳糖化海藻酸钠-壳低聚糖微胶囊的结构和性能，然后将这种微胶囊用于肝细胞包裹，研究肝细胞的形态和功能表达。 　　方法：激光共聚焦显微镜观察半乳糖化海藻酸钠含量分别为50%，30%时，半乳糖化海藻酸钠-壳低聚糖微胶囊的膜结构和膜厚，机械破碎百分率测定微胶囊的力学性能，通过包裹FITC标记的白蛋白和球蛋白的释放来测定微胶囊通透性。倒置相差显微镜观察肝细胞在半乳糖化海藻酸钠-壳低聚糖微胶囊中的形态，检测肝细胞白蛋白和尿素的合成。 　　结果与结论：半乳糖化海藻酸钠-壳低聚糖微胶囊的膜内层致密而外层疏松，呈一种非对称膜的结构。随着半乳糖化海藻酸钠含量的增加，微胶囊的膜也越来越疏松，这些现象表明半乳糖的引入削弱了海藻酸钠分子链上负电荷的密度，影响了静电复合反应。微胶囊的力学性能与胶囊膜的结构和厚度有关；随着半乳糖化海藻酸钠含量的增加，膜结构越来越疏松，同时膜的厚度也减小，因此其力学性能变差。微胶囊的通透性主要决定于皮层的孔径，而疏松的亚层结构对通透性影响不大。制备的微胶囊具有选择性通过白蛋白，截留球蛋白的能力，表明胶囊膜的皮层孔径介于白蛋白和球蛋白尺寸之间。肝细胞在半乳糖化微胶囊中呈球形聚集体形态，并且肝细胞在半乳糖化微胶囊中的白蛋白分泌和尿素合成能力明显强于在海藻酸钠-壳低聚糖微胶囊中的效果。
배경：전기실험성공제비료일충신형반유당화해조산납-각저취당미효낭，국내외상미견이각저취당화해조산납제비적미효낭용우간세포포과적연구。 　　목적：연구반유당화해조산납-각저취당미효낭적결구화성능，연후장저충미효낭용우간세포포과，연구간세포적형태화공능표체。 　　방법：격광공취초현미경관찰반유당화해조산납함량분별위50%，30%시，반유당화해조산납-각저취당미효낭적막결구화막후，궤계파쇄백분솔측정미효낭적역학성능，통과포과FITC표기적백단백화구단백적석방래측정미효낭통투성。도치상차현미경관찰간세포재반유당화해조산납-각저취당미효낭중적형태，검측간세포백단백화뇨소적합성。 　　결과여결론：반유당화해조산납-각저취당미효낭적막내층치밀이외층소송，정일충비대칭막적결구。수착반유당화해조산납함량적증가，미효낭적막야월래월소송，저사현상표명반유당적인입삭약료해조산납분자련상부전하적밀도，영향료정전복합반응。미효낭적역학성능여효낭막적결구화후도유관；수착반유당화해조산납함량적증가，막결구월래월소송，동시막적후도야감소，인차기역학성능변차。미효낭적통투성주요결정우피층적공경，이소송적아층결구대통투성영향불대。제비적미효낭구유선택성통과백단백，절류구단백적능력，표명효낭막적피층공경개우백단백화구단백척촌지간。간세포재반유당화미효낭중정구형취집체형태，병차간세포재반유당화미효낭중적백단백분비화뇨소합성능력명현강우재해조산납-각저취당미효낭중적효과。
BACKGROUND:A novel galactosylated alginate-chitosan oligomer microcapsule has been prepared successful y. There is no report on hepatocytes encapsulated into the microcapsule prepared with chitosan oligomer and alginate sodium. OBJECTIVE:To study the structure and properties of the previous novel galactosylated alginate-chitosan oligomer microcapsule, and then explore the morphology and function expression of the hepatocytes encapsulated. METHODS:The membrane structure and thickness of the microcapsule (containing 50%or 30%galactosylated alginate) were observed using a laser confocal microscopy. Mechanical property was determined by mechanical rupture rate. Permeability was evaluated by release profile of fluoresceine isothiocyanate-labeled human serum albumin and immunoglobulin G. The morphology of hepatocytes in the microcapsule was observed using an inverted phase contrast microscopy. Function expression of the hepatocytes included albumin secretion and urea synthesis. RESULTS AND CONCLUSION:The microcapsule had an asymmetry structure, with dense inner and loosened outer surfaces. With the increase of the galactosylated alginate, the membrane became loose, which indicated the negative charge on the alginate molecular chains was weakened after introduction of galactose, and thus electrostatic complex was affected. Mechanical property was correlated with both membrane structure and thickness. With the increase of the galactosylated alginate, the membrane structure became loose and the thickness was decreased, resulting in poor mechanical properties. The permeability was dependent mainly on the pore size of the skin layer of the membrane other than the loose sublayer. The prepared microcapsule can selectively pass through the human serum albumin and cut off immunoglobulin G, indicating skin pore size between human serum albumin and immunoglobulin G. The hepatocytes can form sphere assemble in the galactosylated alginate-chitosan oligomer microcapsule and exhibit improved albumin secretion and urea synthesis compared to the control in the alginate-chitosan oligomer microcapsule.