CAJ | 학술논문

背景：小肠黏膜下层有抗微生物活性、良好的生物相容性及生物力学性能，能在体内快速降解，与半月板纤维软骨细胞的胞外基质相近。 　　目的：观察小肠黏膜下层与滑膜间充质干细胞是否有良好的组织相容性。 　　方法：先后采用物理方法与化学方法处理猪小肠黏膜下层，并进行苏木精-伊红染色和扫描电镜观察。制备小肠黏膜下层浸提液，行以下实验：①热源实验：在新西兰大白兔耳缘静脉注射小肠黏膜下层浸提液。②皮肤致敏实验：在新西兰大白兔背部皮内分别注射小肠黏膜下层浸提液、多聚甲醛溶液及生理盐水。③全身毒性实验：在新西兰大白兔耳缘静脉分别注射小肠黏膜下层浸提液与生理盐水。将小肠黏膜下层与成骨诱导的兔滑膜间充质干细胞共培养，以小肠黏膜下层单独培养为对照。 　　结果与结论：经物理处理过的小肠黏膜下层表面仍然存在小肠黏膜上皮细胞、脂肪细胞和一些其他细胞黏附；经化学方法处理后其残留的细胞数量明显减少，但主要结构和成分未被改变。小肠黏膜下层黏膜面较肌层面光滑，无细胞残留，表面纤维组织交错形成疏松的三维网状立体结构，孔隙率为80%。小肠黏膜下层是一种无毒、无刺激、无免疫原性，生物相容性很好的生物材料，与兔滑膜间充质干细胞具有良好的组织相容性。
배경：소장점막하층유항미생물활성、량호적생물상용성급생물역학성능，능재체내쾌속강해，여반월판섬유연골세포적포외기질상근。 　　목적：관찰소장점막하층여활막간충질간세포시부유량호적조직상용성。 　　방법：선후채용물리방법여화학방법처리저소장점막하층，병진행소목정-이홍염색화소묘전경관찰。제비소장점막하층침제액，행이하실험：①열원실험：재신서란대백토이연정맥주사소장점막하층침제액。②피부치민실험：재신서란대백토배부피내분별주사소장점막하층침제액、다취갑철용액급생리염수。③전신독성실험：재신서란대백토이연정맥분별주사소장점막하층침제액여생리염수。장소장점막하층여성골유도적토활막간충질간세포공배양，이소장점막하층단독배양위대조。 　　결과여결론：경물리처리과적소장점막하층표면잉연존재소장점막상피세포、지방세포화일사기타세포점부；경화학방법처리후기잔류적세포수량명현감소，단주요결구화성분미피개변。소장점막하층점막면교기층면광활，무세포잔류，표면섬유조직교착형성소송적삼유망상입체결구，공극솔위80%。소장점막하층시일충무독、무자격、무면역원성，생물상용성흔호적생물재료，여토활막간충질간세포구유량호적조직상용성。
BACKGROUND:Smal intestinal submucosa is characterized as antimicrobial activity, good biocompatibility, bio-mechanical properties, and rapid degradation in vivo, similar to the extracellular matrix of meniscal fibrochondrocytes. OBJECTIVE:To observe whether there exists a good histocompatibility between smal intestinal submucosa and synovial mesenchymal stem cells. METHODS:Smal intestinal submucosa was treated with physical and chemical treatment. And hematoxylin-eosin staining and scanning electron microscopy observation were performed. Then, smal intestinal submucosa extracts were prepared for the fol owing experiments. (1) Pyrogenic test:smal intestinal submucosa extracts were injected into the ear vein of New Zealand white rabbits. (2) Skin sensitization test:smal intestinal submucosa extracts, paraformaldehyde solution and normal saline were respectively injected intradermal y into New Zealand white rabbits. (3) General toxicity test:smal intestinal submucosa extracts and normal saline were respectively injected into the ear vein of New Zealand white rabbits. Smal intestinal submucosa was co-cultured with osteogenic rabbit synovial mesenchymal stem cells, and smal intestinal submucosa cultured alone served as control. RESULTS AND CONCLUSION:There were some intestinal mucosal epithelial cells, fat cells and other cells adhered onto the surface of smal intestinal submucosa after physical treatment. While, the amount of residual cells decreased sharply after chemical treatment. But the main structure and the component had not been changed. The surface of smal intestinal submucosa was smooth and no cells remained, and there was a three-dimension network spatial structure. The porosity was 80%. Smal intestinal submucosa is a non-toxic, nonirritating, non-immunogenic biomaterial with very good biocompatibility, which has a good histocompatibility with rabbit synovial mesenchymal stem cells.